BRENDA - Enzyme Database show
show all sequences of 1.3.7.1

Nicotinic acid metabolism. IV. Ferredoxin-dependent reduction of 6-hydroxynicotinic acid to 6-oxo-1,4,5,6-tetrahydronicotinic acid

Holcenberg, J.S.; Tsai, L.; J. Biol. Chem. 244, 1204-1211 (1969)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
arsenate
crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity
Clostridium sp.
phosphate
crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity
Clostridium sp.
pyruvate
crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity
Clostridium sp.
General Stability
General Stability
Organism
cobinamide cyanide, 0.01 mM, accelerates loss of activity in air
Clostridium sp.
ethylene glycol high concentrations stabilize
Clostridium sp.
glycerol, concentrations above 30% stabilize
Clostridium sp.
Na2S * 9 H2O stabilizes, maximal effect at 0.03%
Clostridium sp.
stable to freezing and thawing
Clostridium sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
Ferredoxin
-
Clostridium sp.
0.3
-
methyl viologen
-
Clostridium sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium sp.
-
-
-
Oxidation Stability
Oxidation Stability
Organism
air, 5°C, 24 h, 50% loss of activity
Clostridium sp.
Purification (Commentary)
Commentary
Organism
partial
Clostridium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.9
-
-
Clostridium sp.
Storage Stability
Storage Stability
Organism
several months in liquid nitrogen and up to 50 h with argon
Clostridium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4,5,6-tetrahydro-6-oxonicotinate + oxidized ferredoxin
oxidized methyl viologen is also substrate
391150
Clostridium sp.
6-hydroxynicotinate + reduced ferredoxin
-
391150
Clostridium sp.
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Clostridium sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5
-
stable in liquid nitrogen, unstable in air
Clostridium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Clostridium sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
8.2
pH 5.5: about 60% of activity maximum, pH 8.2: about 20% of activity maximum, rapid decline in activity below pH 5.6 may be partly due to destruction of ferredoxin in acid
Clostridium sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
-
Clostridium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
CoA
crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity
Clostridium sp.
Ferredoxin
reduced or oxidized, as electron carrier
Clostridium sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
arsenate
crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity
Clostridium sp.
phosphate
crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity
Clostridium sp.
pyruvate
crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity
Clostridium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
CoA
crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity
Clostridium sp.
Ferredoxin
reduced or oxidized, as electron carrier
Clostridium sp.
General Stability (protein specific)
General Stability
Organism
cobinamide cyanide, 0.01 mM, accelerates loss of activity in air
Clostridium sp.
ethylene glycol high concentrations stabilize
Clostridium sp.
glycerol, concentrations above 30% stabilize
Clostridium sp.
Na2S * 9 H2O stabilizes, maximal effect at 0.03%
Clostridium sp.
stable to freezing and thawing
Clostridium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
Ferredoxin
-
Clostridium sp.
0.3
-
methyl viologen
-
Clostridium sp.
Oxidation Stability (protein specific)
Oxidation Stability
Organism
air, 5°C, 24 h, 50% loss of activity
Clostridium sp.
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Clostridium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.9
-
-
Clostridium sp.
Storage Stability (protein specific)
Storage Stability
Organism
several months in liquid nitrogen and up to 50 h with argon
Clostridium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4,5,6-tetrahydro-6-oxonicotinate + oxidized ferredoxin
oxidized methyl viologen is also substrate
391150
Clostridium sp.
6-hydroxynicotinate + reduced ferredoxin
-
391150
Clostridium sp.
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Clostridium sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5
-
stable in liquid nitrogen, unstable in air
Clostridium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Clostridium sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
8.2
pH 5.5: about 60% of activity maximum, pH 8.2: about 20% of activity maximum, rapid decline in activity below pH 5.6 may be partly due to destruction of ferredoxin in acid
Clostridium sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
-
Clostridium sp.
Other publictions for EC 1.3.7.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670692
Alhapel
Molecular and functional analy ...
Eubacterium barkeri
Proc. Natl. Acad. Sci. USA
103
12341-12346
2006
-
-
-
-
-
-
-
-
-
1
1
-
-
4
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391151
Kitts
Elucidation of the complete Az ...
Azorhizobium caulinodans
J. Bacteriol.
174
7791-7797
1992
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391150
Holcenberg
Nicotinic acid metabolism. IV. ...
Clostridium sp.
J. Biol. Chem.
244
1204-1211
1969
3
-
-
-
-
5
-
2
-
-
-
-
-
1
1
-
1
-
-
-
1
1
1
-
1
-
1
-
1
1
1
2
-
-
-
3
-
-
2
-
-
5
-
-
-
2
-
-
-
-
-
1
-
1
-
-
1
1
1
-
1
-
1
-
1
1
1
-
-
-
-
-
-
-