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Literature summary for 1.3.5.6 extracted from

  • Breitenbach, J.; Bruns, M.; Sandmann, G.
    Contribution of two zeta-carotene desaturases to the poly-cis desaturation pathway in the cyanobacterium Nostoc PCC 7120 (2013), Arch. Microbiol., 195, 491-498.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene crtQa, expressionin Escherichia coli, gene crtQb, expression of CrtQB in Escherichia coli strain JM101 Nostoc sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7,9,9'-tricis-neurosporene + a quinone Nostoc sp. catalyzed by zeta-carotene desaturase CrtQa, very poorly by CrtQb 7,9,7',9'-tetracis-lycopene + a quinol
-
?
7,9,9'-tricis-neurosporene + a quinone Nostoc sp. PCC 7120 catalyzed by zeta-carotene desaturase CrtQa, very poorly by CrtQb 7,9,7',9'-tetracis-lycopene + a quinol
-
?
9,9'-dicis-zeta-carotene + a quinone Nostoc sp. catalyzed by zeta-carotene desaturases CrtQa and CrtQb 7,9,9'-tricis-neurosporene + a quinol
-
?
9,9'-dicis-zeta-carotene + a quinone Nostoc sp. PCC 7120 catalyzed by zeta-carotene desaturases CrtQa and CrtQb 7,9,9'-tricis-neurosporene + a quinol
-
?
additional information Nostoc sp. strain PCC 7120 contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, which differ in their preferred utilization of zeta-carotene Z isomers. CrtQa con­verts zeta-carotene isomers that are poorly metabolized by CrtQb and CrtQa still possesses the Z to E isomerase function of the ancestral desaturase CrtI. CrtQb is an enzyme with one molecule of tightly bound FAD and acts as a dehydrogenase transferring hydrogen to oxidized plastoquinone. CrtQb yields only trace amounts of 7,9-Z neurosporene and 7,9,7',9'-Z lycopene ?
-
?
additional information Nostoc sp. PCC 7120 strain PCC 7120 contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, which differ in their preferred utilization of zeta-carotene Z isomers. CrtQa con­verts zeta-carotene isomers that are poorly metabolized by CrtQb and CrtQa still possesses the Z to E isomerase function of the ancestral desaturase CrtI. CrtQb is an enzyme with one molecule of tightly bound FAD and acts as a dehydrogenase transferring hydrogen to oxidized plastoquinone. CrtQb yields only trace amounts of 7,9-Z neurosporene and 7,9,7',9'-Z lycopene ?
-
?

Organism

Organism UniProt Comment Textmining
Nostoc sp.
-
contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, encoded by genes crtQa and crtQb
-
Nostoc sp. PCC 7120
-
contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, encoded by genes crtQa and crtQb
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,9,9'-tricis-neurosporene + a quinone catalyzed by zeta-carotene desaturase CrtQa, very poorly by CrtQb Nostoc sp. 7,9,7',9'-tetracis-lycopene + a quinol
-
?
7,9,9'-tricis-neurosporene + a quinone catalyzed by zeta-carotene desaturase CrtQa, very poorly by CrtQb Nostoc sp. PCC 7120 7,9,7',9'-tetracis-lycopene + a quinol
-
?
9,9'-dicis-zeta-carotene + a quinone catalyzed by zeta-carotene desaturases CrtQa and CrtQb Nostoc sp. 7,9,9'-tricis-neurosporene + a quinol
-
?
9,9'-dicis-zeta-carotene + a quinone catalyzed by zeta-carotene desaturases CrtQa and CrtQb Nostoc sp. PCC 7120 7,9,9'-tricis-neurosporene + a quinol
-
?
additional information strain PCC 7120 contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, which differ in their preferred utilization of zeta-carotene Z isomers. CrtQa con­verts zeta-carotene isomers that are poorly metabolized by CrtQb and CrtQa still possesses the Z to E isomerase function of the ancestral desaturase CrtI. CrtQb is an enzyme with one molecule of tightly bound FAD and acts as a dehydrogenase transferring hydrogen to oxidized plastoquinone. CrtQb yields only trace amounts of 7,9-Z neurosporene and 7,9,7',9'-Z lycopene Nostoc sp. ?
-
?
additional information zeta-carotene desaturase CrtQb converts (9Z)-zeta-carotene efficiently into (7Z,9Z)-neurosporene but stops at this stage without substantial lycopene formation. (all-E)-zeta-carotene cannot be converted by CrtQb. Similarly to (9Z)-zeta-carotene, the (all-E)-isomer is efficiently converted by CrtQa, substrate specificities, overview Nostoc sp. ?
-
?
additional information strain PCC 7120 contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, which differ in their preferred utilization of zeta-carotene Z isomers. CrtQa con­verts zeta-carotene isomers that are poorly metabolized by CrtQb and CrtQa still possesses the Z to E isomerase function of the ancestral desaturase CrtI. CrtQb is an enzyme with one molecule of tightly bound FAD and acts as a dehydrogenase transferring hydrogen to oxidized plastoquinone. CrtQb yields only trace amounts of 7,9-Z neurosporene and 7,9,7',9'-Z lycopene Nostoc sp. PCC 7120 ?
-
?
additional information zeta-carotene desaturase CrtQb converts (9Z)-zeta-carotene efficiently into (7Z,9Z)-neurosporene but stops at this stage without substantial lycopene formation. (all-E)-zeta-carotene cannot be converted by CrtQb. Similarly to (9Z)-zeta-carotene, the (all-E)-isomer is efficiently converted by CrtQa, substrate specificities, overview Nostoc sp. PCC 7120 ?
-
?

Synonyms

Synonyms Comment Organism
CrtQa
-
Nostoc sp.
CrtQb
-
Nostoc sp.
zeta-carotene desaturase
-
Nostoc sp.

Cofactor

Cofactor Comment Organism Structure
FAD tightly bound to zeta-carotene desaturase CrtQb Nostoc sp.