BRENDA - Enzyme Database
show all sequences of 1.3.5.6

Alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution

Wang, C.W.; Liao, J.C.; J. Biol. Chem. 276, 41161-41164 (2001)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
E508G
random mutagenesis, the mutation has little effect on the enzyme activity
Rhodobacter sphaeroides
F166I
random mutagenesis, a beneficial mutation that helps the conversion of product from neurosporene to lycopene
Rhodobacter sphaeroides
F220S
random mutagenesis, the mutation has little effect on the enzyme activity
Rhodobacter sphaeroides
H12Q
random mutagenesis, the mutation has little effect on the enzyme activity
Rhodobacter sphaeroides
L148H
random mutagenesis, the beneficial mutation is highly increasing the production of lycopene to over 80%
Rhodobacter sphaeroides
M402T
random mutagenesis, the mutation reduces the lycopene production rate
Rhodobacter sphaeroides
additional information
alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution combining random mutagenesis and site-directed mutagenesis, DNA sequence determinations. Beneficial mutations increase the percent of lycopene production to 90%; introduction of stop mutations, M1, M2 and M3
Rhodobacter sphaeroides
V68D
random mutagenesis, a beneficial mutation that helps the conversion of product from neurosporene to lycopene
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phytoene + quinone
Rhodobacter sphaeroides
via phytofluene and zeta-carotene
7,9,9'-tricis-neurosporene + quinol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter sphaeroides
-
gene crtI
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7,9,9'-tricis-neurosporene + a quinone
mutant activity
712351
Rhodobacter sphaeroides
7,9,7',9'-tetracis-lycopene + a quinol
-
-
-
?
phytoene + quinone
via phytofluene and zeta-carotene
712351
Rhodobacter sphaeroides
7,9,9'-tricis-neurosporene + quinol
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E508G
random mutagenesis, the mutation has little effect on the enzyme activity
Rhodobacter sphaeroides
F166I
random mutagenesis, a beneficial mutation that helps the conversion of product from neurosporene to lycopene
Rhodobacter sphaeroides
F220S
random mutagenesis, the mutation has little effect on the enzyme activity
Rhodobacter sphaeroides
H12Q
random mutagenesis, the mutation has little effect on the enzyme activity
Rhodobacter sphaeroides
L148H
random mutagenesis, the beneficial mutation is highly increasing the production of lycopene to over 80%
Rhodobacter sphaeroides
M402T
random mutagenesis, the mutation reduces the lycopene production rate
Rhodobacter sphaeroides
additional information
alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution combining random mutagenesis and site-directed mutagenesis, DNA sequence determinations. Beneficial mutations increase the percent of lycopene production to 90%; introduction of stop mutations, M1, M2 and M3
Rhodobacter sphaeroides
V68D
random mutagenesis, a beneficial mutation that helps the conversion of product from neurosporene to lycopene
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phytoene + quinone
Rhodobacter sphaeroides
via phytofluene and zeta-carotene
7,9,9'-tricis-neurosporene + quinol
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7,9,9'-tricis-neurosporene + a quinone
mutant activity
712351
Rhodobacter sphaeroides
7,9,7',9'-tetracis-lycopene + a quinol
-
-
-
?
phytoene + quinone
via phytofluene and zeta-carotene
712351
Rhodobacter sphaeroides
7,9,9'-tricis-neurosporene + quinol
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosporene, whereas the rest produce lycopene. These two types of enzymes, although similar in function, have relatively low similarity, below 60%, in terms of nucleotide or amino acid sequence. Phytoene desaturase encoded by gene crtI is a neurosporene-producing enzyme
Rhodobacter sphaeroides
physiological function
phytoene desaturase encoded by crtI catalyzes the desaturation of phytoene in the carotenoid biosynthesis pathway, approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosporene, whereas the rest produce lycopene, mechanism controlling the product specificity, phytoene desaturase encoded by gene crtI is a neurosporene-producing enzyme, overview
Rhodobacter sphaeroides
General Information (protein specific)
General Information
Commentary
Organism
evolution
approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosporene, whereas the rest produce lycopene. These two types of enzymes, although similar in function, have relatively low similarity, below 60%, in terms of nucleotide or amino acid sequence. Phytoene desaturase encoded by gene crtI is a neurosporene-producing enzyme
Rhodobacter sphaeroides
physiological function
phytoene desaturase encoded by crtI catalyzes the desaturation of phytoene in the carotenoid biosynthesis pathway, approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosporene, whereas the rest produce lycopene, mechanism controlling the product specificity, phytoene desaturase encoded by gene crtI is a neurosporene-producing enzyme, overview
Rhodobacter sphaeroides
Other publictions for EC 1.3.5.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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712351
Wang
Alteration of product specific ...
Rhodobacter sphaeroides
J. Biol. Chem.
276
41161-41164
2001
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8
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