Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Oryza sativa Indica Group |
Crystallization (Comment) | Organism |
---|---|
low-resolution model, homotetrameric arrangement of the protein | Oryza sativa Indica Group |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
norflurazon | arrests the reaction by stabilizing the intermediary FADred. An excess of the quinone acceptor relieves this blockage | Oryza sativa Indica Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated to | Oryza sativa Indica Group | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
450000 | - |
gel filtration | Oryza sativa Indica Group |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Indica Group | A2XDA1 | - |
- |
Oxidation Stability | Organism |
---|---|
reduced form of protein is stable for over 2 h in ambient oxygen atmosphere, rapid reoxidation can be achieved with excess decyl-plastoquinone | Oryza sativa Indica Group |
Purification (Comment) | Organism |
---|---|
recombinant protein. High-order oligomers formed in solution are the basis for an active preparation | Oryza sativa Indica Group |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + 2 decyl-plastoquinone | - |
Oryza sativa Indica Group | 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol | - |
? | |
additional information | benzoquinone cosubstrates are strongly preferred over naphthoquinones | Oryza sativa Indica Group | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 56200, calculated, 8 * 56000, SDS-PAGE, recombinant protein with His-tag | Oryza sativa Indica Group |
Synonyms | Comment | Organism |
---|---|---|
PDS1 | - |
Oryza sativa Indica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | sole protein-bound redox-cofactor | Oryza sativa Indica Group |