Cloned (Comment) | Organism |
---|---|
heterologous expression in a strain of Escherichia coli containing a carotenoid-producing plasmid | Brassica oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the enzyme from stroma is in an approximately 660000 Da complex | Brassica oleracea | 9507 | - |
chromoplast | BoPDS is present at similar levels in chromoplasts and leucoplasts | Brassica oleracea | 9509 | - |
leucoplast | BoPDS is present at similar levels in chromoplasts and leucoplasts | Brassica oleracea | 9516 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE, the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex | Brassica oleracea |
350000 | - |
the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex | Brassica oleracea |
660000 | - |
the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex | Brassica oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + acceptor | Brassica oleracea | phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway | 9,15,9'-tricis-zeta-carotene + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | B2X8P6 | var. botrytis, white cultivar Stovepipe | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme form from stroma appears to be slightly larger in size than the membrane version, which may be because of different posttranslational modifications of BoPDS in these two locations | Brassica oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | BoPDS has the highest level of expression in flowers with an approximate 4.5fold increase of BoPDS transcript level compared with leaf tissue | Brassica oleracea | - |
leaf | BoPDS has the highest level of expression in flowers with an approximate 4.5fold increase of BoPDS transcript level compared with leaf tissue | Brassica oleracea | - |
root | siliques and roots show the lowest levels of expression | Brassica oleracea | - |
seed | - |
Brassica oleracea | - |
silique | siliques and roots show the lowest levels of expression | Brassica oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + acceptor | - |
Brassica oleracea | 9,15,9'-tricis-zeta-carotene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway | Brassica oleracea | 9,15,9'-tricis-zeta-carotene + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE, the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex | Brassica oleracea |
Synonyms | Comment | Organism |
---|---|---|
BoPDS | - |
Brassica oleracea |