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Literature summary for 1.3.5.2 extracted from

  • Shi, J.; Palfey, B.A.; Dertouzos, J.; Jensen, K.F.; Gafni, A.; Steel, D.
    Multiple states of the Tyr318Leu mutant of dihydroorotate dehydrogenase revealed by single-molecule kinetics (2004), J. Am. Chem. Soc., 126, 6914-6922.
    View publication on PubMed
Search for more literature for 1.3.5.2

Protein Variants

Protein Variants Comment Organism
Y318L confocal fluorescence spectroscopy study Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated to Escherichia coli 16020
-

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + 2,6-dihydrophenolindophenol
-
 Escherichia coli orotate + reduced 2,6-dihydrophenolindophenol
-
 r
L-dihydroorotate + 2,6-dichlorophenolindophenol
-
 Escherichia coli orotate + reduced 2,6-dichlorophenolindophenol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.7
-
 (S)-dihydroorotate mutant Y318L, 22°C, pH 7.0 Escherichia coli
9.3
-
 (S)-dihydroorotate mutant Y318L, 22°C, pH 7.0, presence of 0.1% TritonX-100 Escherichia coli