Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.5.2 extracted from

  • Ullrich, A.; Knecht, W.; Fries, M.; Loffler, M.
    Recombinant expression of N-terminal truncated mutants of the membrane bound mouse, rat and human flavoenzyme dihydroorotate dehydrogenase. A versatile tool to rate inhibitor effects? (2001), Eur. J. Biochem., 268, 1861-1868.
    View publication on PubMed
Search for more literature for 1.3.5.2

Application

Application Comment Organism
medicine great interest in inhibitors as potential therapeutic agents for the treatment of diseases involving aberrant cell proliferation Mus musculus
medicine great interest in inhibitors as potential therapeutic agents for the treatment of diseases involving aberrant cell proliferation Homo sapiens
medicine great interest in inhibitors as potential therapeutic agents for the treatment of diseases involving aberrant cell proliferation Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL-1 Blue, histidine tagged Mus musculus
expressed in Escherichia coli XL-1 Blue, histidine tagged Homo sapiens
expressed in Escherichia coli XL-1 Blue, histidine tagged Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
A77 1726 malononitrilamide, 50% inhibition at 18-773 nM Homo sapiens
A77 1726 malononitrilamide, 50% inhibition at 18-773 nM Mus musculus
A77 1726 malononitrilamide, 50% inhibition at 18-773 nM Rattus norvegicus
brequinar sodium 50% inhibition at 6-127 nM Homo sapiens
brequinar sodium 50% inhibition at 6-127 nM Mus musculus
brequinar sodium 50% inhibition at 6-127 nM Rattus norvegicus
MNA 279 malononitrilamide, 50% inhibition at 22-715 nM Homo sapiens
MNA 279 malononitrilamide, 50% inhibition at 22-715 nM Mus musculus
MNA 279 malononitrilamide, 50% inhibition at 22-715 nM Rattus norvegicus
MNA 715 malononitrilamide, 50% inhibition at 41-109 nM Homo sapiens
MNA 715 malononitrilamide, 50% inhibition at 41-109 nM Mus musculus
MNA 715 malononitrilamide, 50% inhibition at 41-109 nM Rattus norvegicus
redoxal 50% inhibition at 45-88 nM Homo sapiens
redoxal 50% inhibition at 45-88 nM Mus musculus
redoxal 50% inhibition at 45-88 nM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 ubiquinone
-
 Rattus norvegicus
0.01
-
 S-dihydroorotate
-
 Homo sapiens
0.011
-
 S-dihydroorotate
-
 Rattus norvegicus
0.014
-
 ubiquinone
-
 Homo sapiens
0.026
-
 S-dihydroorotate
-
 Mus musculus
0.062
-
 ubiquinone
-
 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion inner membrane Mus musculus 5739
-
mitochondrion inner membrane Homo sapiens 5739
-
mitochondrion inner membrane Rattus norvegicus 5739
-
mitochondrion integral protein Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-dihydroorotate + ubiquinone Mus musculus fourth enzyme in pyrimidine synthesis orotate + ubiquinol
-
 ?
(S)-dihydroorotate + ubiquinone Homo sapiens fourth enzyme in pyrimidine synthesis orotate + ubiquinol
-
 ?
(S)-dihydroorotate + ubiquinone Rattus norvegicus fourth enzyme in pyrimidine synthesis orotate + ubiquinol
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+/nitrilotriacetate column Mus musculus
Ni2+/nitrilotriacetate column Homo sapiens
Ni2+/nitrilotriacetate column Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101
-
-
 Mus musculus
107
-
-
 Homo sapiens
125
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
great inactivation by repeated thawing and freezing Mus musculus
great inactivation by repeated thawing and freezing Homo sapiens
great inactivation by repeated thawing and freezing Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + ubiquinone
-
 Mus musculus orotate + ubiquinol
-
 ?
(S)-dihydroorotate + ubiquinone
-
 Homo sapiens orotate + ubiquinol
-
 ?
(S)-dihydroorotate + ubiquinone
-
 Rattus norvegicus orotate + ubiquinol
-
 ?
(S)-dihydroorotate + ubiquinone fourth enzyme in pyrimidine synthesis Mus musculus orotate + ubiquinol
-
 ?
(S)-dihydroorotate + ubiquinone fourth enzyme in pyrimidine synthesis Homo sapiens orotate + ubiquinol
-
 ?
(S)-dihydroorotate + ubiquinone fourth enzyme in pyrimidine synthesis Rattus norvegicus orotate + ubiquinol
-
 ?

Subunits

Subunits Comment Organism
?
-
 Rattus norvegicus
? x * 43000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
DHODH
-
 Mus musculus
DHODH
-
 Homo sapiens
DHODH
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
71
-
 S-dihydroorotate
-
 Mus musculus
75
-
 S-dihydroorotate
-
 Homo sapiens
95
-
 S-dihydroorotate
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Homo sapiens
8
-
-
 Rattus norvegicus
8 8.1
-
 Mus musculus
8 8.1
-
 Homo sapiens
8 8.1
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
additional information flavoenzyme, 0.6-1.2 mol flavin per mol protein Mus musculus
additional information flavoenzyme, 0.6-1.2 mol flavin per mol protein Homo sapiens
additional information flavoenzyme, 0.6-1.2 mol flavin per mol protein Rattus norvegicus