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Literature summary for 1.3.5.1 extracted from
- The unusual iron sulfur composition of the Acidianus ambivalens succinate dehydrogenase complex (1999), Biochim. Biophys. Acta, 1411, 134-141.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Acidianus ambivalens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
fumarate | pH 6.5, 70°C, detergent treated membranes from aerobic growth | Acidianus ambivalens |  |
| 0.06 | - |
fumarate | pH 6.5, 70°C, determined for activity in membranes from aerobic growth | Acidianus ambivalens | |
| 0.08 | - |
fumarate | pH 6.5, 70°C, detergent treated membranes from anaerobic growth | Acidianus ambivalens | |
| 0.3 | - |
succinate | pH 6.5, 70°C, detergent treated membranes from anaerobic growth | Acidianus ambivalens | |
| 0.5 | - |
succinate | pH 6.5, 70°C, detergent treated membranes from aerobic growth | Acidianus ambivalens | |
| 0.5 | - |
succinate | pH 6.5, 70°C, determined for activity in membranes from aerobic growth | Acidianus ambivalens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Saccharolobus solfataricus | 16020 | - |
| membrane | - |
Acidianus ambivalens | 16020 | - |
| membrane | - |
Acidianus infernus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | SdhB is the iron-sulfur subunit | Acidianus ambivalens | |
| Iron-sulfur cluster | the enzyme has an unusual iron-sulfur cluster composition in respect to that of the canonical succinate dehydrogenases. Center S3, the succinate responsive [3Fe-4S]1+/0 cluster of succinate dehydrogenases, is not present. The remaining centers, clusters S1 ([2Fe-2S]1+/2+) and S2 ([4Fe-4S]2+/1+), can be observed | Saccharolobus solfataricus | |
| Iron-sulfur cluster | the enzyme has an unusual iron-sulfur cluster composition with respect to that of the canonical succinate dehydrogenases. Center S3, the succinate responsive [3Fe-4S]1+/0 cluster of succinate dehydrogenases, is not present in membranes prepared from aerobically grown Acidianus ambivalens, nor in partially purified complex fractions. It is replaced by a second [4Fe-4S] center, by incorporation of an additional cysteine, at the cysteine cluster binding motif (CxxYxxCxxxC-->CxxCxxCxxxC). The remaining centers, clusters S1 ([2Fe-2S]1+/2+) and S2 ([4Fe-4S]2+/1+), can be observed | Acidianus ambivalens | |
| Iron-sulfur cluster | the enzyme has an unusual iron-sulfur cluster composition with respect to that of the canonical succinate dehydrogenases. Center S3, the succinate responsive [3Fe-4S]1+/0 cluster of succinate dehydrogenases, is not present. The remaining centers, clusters S1 ([2Fe-2S]1+/2+) and S2 ([4Fe-4S]2+/1+), can be observed | Acidianus infernus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumarate + reduced acceptor | Acidianus ambivalens | the obligate autotroph requires fumarate reductase activity if it performs CO2 fixation via a reductive citric acid cycle | succinate + acceptor | - |
r | |
| succinate + caldariellaquinone | Acidianus ambivalens | - |
fumarate + caldariellaquinol | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidianus ambivalens | - |
- |
- |
| Acidianus ambivalens | O73937 and O73937 and O73939 and O73940 | O73937: FAD-containing subunit SdhA, O73937: iron-sulfur subunit SdhB, O73939: subunit SdhC, O73940: subunit SdhD | - |
| Acidianus infernus | - |
- |
- |
| Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acidianus ambivalens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:aerobically-grown cell | - |
Acidianus ambivalens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumarate + reduced acceptor | the obligate autotroph requires fumarate reductase activity if it performs CO2 fixation via a reductive citric acid cycle | Acidianus ambivalens | succinate + acceptor | - |
r | |
| fumarate + reduced benzyl viologen | - |
Acidianus ambivalens | succinate + oxidized benzyl viologen | - |
r | |
| succinate + caldariellaquinone | - |
Acidianus ambivalens | fumarate + caldariellaquinol | - |
r | |
| succinate + oxidized 2,6-dichlorophenolindophenol | - |
Acidianus ambivalens | fumarate + reduced 2,6-dichlorophenolindophenol | - |
r | |
| succinate + oxidized benzyl viologen | - |
Acidianus ambivalens | fumarate + reduced benzyl viologen | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Acidianus ambivalens |
| 75 | 81 | from 30°C to 75°C the activity increases 45fold, with an optimum temperature between 75°C and 81°C | Acidianus ambivalens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 75 | from 30°C to 75°C the activity increases 45fold, with an optimum temperature between 75°C and 81°C | Acidianus ambivalens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Acidianus ambivalens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | SdhA is the FAD-containing subunit | Acidianus ambivalens | |
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