Cloned (Comment) | Organism |
---|---|
sdhB from mutant H229Y is ligated into a cosmid, pUFR034, to generate pUFR034RAX, which confers resistance to amicarthiazol when transformed into the wild-type sensitive strain | Xanthomonas oryzae pv. oryzae |
Protein Variants | Comment | Organism |
---|---|---|
H229Y | the single amino acid substitution in the SdhB protein of succinate dehydrogenase determines resistance to amicarthiazol, the mutant is insensitive to the fungicide | Xanthomonas oryzae pv. oryzae |
additional information | several resistant mutant strains, the SDH activity of mutant strain XRUVI is clearly lower than that of wild-type strain ZJ173 in the absence of amicarthiazol, overview | Xanthomonas oryzae pv. oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amicarthiazol | i.e. 2-amino-4-methylthiazole -5-carboxanilide, a systemic fungicide. The wild-type enzyme is strongly inhibited by amicarthiazol, while that in resistant mutants is insensitive. A single amino acid substitution H229Y in the SdhB protein of succinate dehydrogenase determines resistance to amicarthiazol, molecular resistance mechanism, overview; i.e. 2-amino-4-methylthiazole-5-carboxanilide, a systemic fungicide. The wild-type enzyme is strongly inhibited by amicarthiazol, while that in resistant mutants is insensitive. A single amino acid substitution H229Y in the SdhB protein of succinate dehydrogenase determines resistance to amicarthiazol, molecular resistance mechanism, overview | Xanthomonas oryzae pv. oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Xanthomonas oryzae pv. oryzae | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate + FAD | Xanthomonas oryzae pv. oryzae | - |
fumarate + FADH2 | - |
? | |
succinate + FAD | Xanthomonas oryzae pv. oryzae ZJ173 | - |
fumarate + FADH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas oryzae pv. oryzae | Q6DLZ2 | subunit SdhB | - |
Xanthomonas oryzae pv. oryzae | Q6DLZ3 | subunit SdhA | - |
Xanthomonas oryzae pv. oryzae ZJ173 | Q6DLZ2 | subunit SdhB | - |
Xanthomonas oryzae pv. oryzae ZJ173 | Q6DLZ3 | subunit SdhA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate + FAD | - |
Xanthomonas oryzae pv. oryzae | fumarate + FADH2 | - |
? | |
succinate + FAD | - |
Xanthomonas oryzae pv. oryzae ZJ173 | fumarate + FADH2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | succinate dehydrogenase forms the peripheral part of the succinate-ubiquinone oxidoreductase, EC 1.3.5.1, and is composed of a flavoprotein, SdhA, and an iron-sulfur protein, SdhB | Xanthomonas oryzae pv. oryzae |
Synonyms | Comment | Organism |
---|---|---|
SDH | - |
Xanthomonas oryzae pv. oryzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Xanthomonas oryzae pv. oryzae |