BRENDA - Enzyme Database show
show all sequences of 1.3.3.8

Separation and characterization of cytokinin-inducible (S)-tetrahydroberberine oxidases controlling berberine biosynthesis in Thalictrum minus cell cultures

Hara, M.; Morio, H.; Yazaki, K.; Tanaka, S.; Tabata, M.; Phytochemistry 38, 89-93 (1995)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0021
-
(S)-tetrahydroberberine
isoenzyme I
Thalictrum minus var. hypoleucum
0.0065
-
(S)-tetrahydroberberine
-
Coptis japonica
0.0106
-
(S)-tetrahydroberberine
isoenzyme II
Thalictrum minus var. hypoleucum
0.0267
-
(S)-tetrahydroberberine
-
Berberis stolonifera
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
3 * 46000, isoenzyme II, gel filtration
Thalictrum minus var. hypoleucum
58000
-
3 * 58000
Coptis japonica
100000
-
gel filtration
Berberis stolonifera
145000
-
isoenzyme II, gel filtration
Thalictrum minus var. hypoleucum
155000
-
gel filtration
Coptis japonica
200000
-
higher than, isoenzyme I, gel filtration
Thalictrum minus var. hypoleucum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Berberis stolonifera
-
-
-
Coptis japonica
-
Makino var. dissecta Nakai
-
Thalictrum dipterocarpum Franch.
-
-
-
Thalictrum flavum ssp. glaucum
-
-
-
Thalictrum minus var. hypoleucum
-
native to Japan
-
Purification (Commentary)
Commentary
Organism
copurification of three isoforms by ion-exchange
Thalictrum minus var. hypoleucum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Thalictrum minus var. hypoleucum
-
cell culture
-
Coptis japonica
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-tetrahydroberberine + O2
-
391078
Berberis stolonifera
berberine + H2O2 + H2O
-
391078
Berberis stolonifera
?
(S)-tetrahydroberberine + O2
-
391078
Coptis japonica
berberine + H2O2 + H2O
-
391078
Coptis japonica
?
(S)-tetrahydroberberine + O2
-
391078
Thalictrum minus var. hypoleucum
berberine + H2O2 + H2O
-
391078
Thalictrum minus var. hypoleucum
?
(S,R)-tetrahydrojatrorrhizine + O2
-
391078
Berberis stolonifera
jatrorrhizine + H2O2
-
391078
Berberis stolonifera
?
(S,R)-tetrahydrojatrorrhizine + O2
-
391078
Coptis japonica
jatrorrhizine + H2O2
-
391078
Coptis japonica
?
(S,R)-tetrahydrojatrorrhizine + O2
-
391078
Thalictrum flavum ssp. glaucum
jatrorrhizine + H2O2
-
391078
Thalictrum flavum ssp. glaucum
?
(S,R)-tetrahydrojatrorrhizine + O2
-
391078
Thalictrum dipterocarpum Franch.
jatrorrhizine + H2O2
-
391078
Thalictrum dipterocarpum Franch.
?
Subunits
Subunits
Commentary
Organism
trimer
3 * 58000
Coptis japonica
trimer
3 * 46000, isoenzyme II, gel filtration
Thalictrum minus var. hypoleucum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Coptis japonica
8.9
-
-
Berberis stolonifera
9
-
-
Thalictrum minus var. hypoleucum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0021
-
(S)-tetrahydroberberine
isoenzyme I
Thalictrum minus var. hypoleucum
0.0065
-
(S)-tetrahydroberberine
-
Coptis japonica
0.0106
-
(S)-tetrahydroberberine
isoenzyme II
Thalictrum minus var. hypoleucum
0.0267
-
(S)-tetrahydroberberine
-
Berberis stolonifera
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
3 * 46000, isoenzyme II, gel filtration
Thalictrum minus var. hypoleucum
58000
-
3 * 58000
Coptis japonica
100000
-
gel filtration
Berberis stolonifera
145000
-
isoenzyme II, gel filtration
Thalictrum minus var. hypoleucum
155000
-
gel filtration
Coptis japonica
200000
-
higher than, isoenzyme I, gel filtration
Thalictrum minus var. hypoleucum
Purification (Commentary) (protein specific)
Commentary
Organism
copurification of three isoforms by ion-exchange
Thalictrum minus var. hypoleucum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Coptis japonica
-
cell culture
-
Thalictrum minus var. hypoleucum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-tetrahydroberberine + O2
-
391078
Berberis stolonifera
berberine + H2O2 + H2O
-
391078
Berberis stolonifera
?
(S)-tetrahydroberberine + O2
-
391078
Coptis japonica
berberine + H2O2 + H2O
-
391078
Coptis japonica
?
(S)-tetrahydroberberine + O2
-
391078
Thalictrum minus var. hypoleucum
berberine + H2O2 + H2O
-
391078
Thalictrum minus var. hypoleucum
?
(S,R)-tetrahydrojatrorrhizine + O2
-
391078
Berberis stolonifera
jatrorrhizine + H2O2
-
391078
Berberis stolonifera
?
(S,R)-tetrahydrojatrorrhizine + O2
-
391078
Coptis japonica
jatrorrhizine + H2O2
-
391078
Coptis japonica
?
(S,R)-tetrahydrojatrorrhizine + O2
-
391078
Thalictrum flavum ssp. glaucum
jatrorrhizine + H2O2
-
391078
Thalictrum flavum ssp. glaucum
?
(S,R)-tetrahydrojatrorrhizine + O2
-
391078
Thalictrum dipterocarpum Franch.
jatrorrhizine + H2O2
-
391078
Thalictrum dipterocarpum Franch.
?
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
3 * 58000
Coptis japonica
trimer
3 * 46000, isoenzyme II, gel filtration
Thalictrum minus var. hypoleucum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Coptis japonica
8.9
-
-
Berberis stolonifera
9
-
-
Thalictrum minus var. hypoleucum
Other publictions for EC 1.3.3.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725930
Chen
Molecular characterization, re ...
Corydalis saxicola
Mol. Biol. Rep.
39
3319-3326
2012
-
-
1
-
-
-
-
-
-
-
2
1
-
5
-
-
-
-
-
5
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
5
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
726136
Matsushima
-
Pathway engineering of benzyli ...
Coptis japonica
Plant Biotechnol.
29
473-481
2012
-
-
1
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
1
-
-
2
1
1
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
2
1
1
-
-
-
1
-
-
1
-
1
1
-
-
-
391078
Hara
-
Separation and characterizatio ...
Berberis stolonifera, Coptis japonica, Thalictrum dipterocarpum Franch., Thalictrum flavum ssp. glaucum, Thalictrum minus var. hypoleucum
Phytochemistry
38
89-93
1995
-
-
-
-
-
-
-
4
-
-
6
-
-
5
-
-
1
-
-
2
-
-
7
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
6
-
-
-
-
1
-
2
-
-
7
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
391079
Nair
-
Partial purification and modul ...
Coscinium fenestratum
Plant Sci.
96
217-221
1994
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391076
Kobayashi
-
The role of ethylene in berber ...
Thalictrum minus var. hypoleucum
Phytochemistry
30
3605-3609
1991
1
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391077
Okada
Isolation, sequence, and bacte ...
Coptis japonica
Proc. Natl. Acad. Sci. USA
86
534-538
1989
-
-
1
-
-
-
-
-
-
-
1
-
-
6
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
391075
Okada
-
Purification and characterizat ...
Coptis japonica
Phytochemistry
27
979-982
1988
-
-
1
-
-
1
1
1
-
-
2
1
-
1
-
-
1
-
-
1
1
1
2
1
1
-
3
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
1
-
-
2
1
-
-
-
1
-
1
1
1
2
1
1
-
3
-
1
-
-
-
-
-
-
-
-
-
391074
Amann
-
(S)-Tetrahydroprotoberberine o ...
Berberis wilsoniae, Papaver somniferum
Tetrahedron Lett.
25
953-954
1984
-
-
-
-
-
-
1
2
-
2
1
1
-
2
-
-
1
-
-
-
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
2
1
1
-
-
-
1
-
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-