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Literature summary for 1.3.3.8 extracted from

  • Okada, N.; Shinmyo, A.; Okada, H.; Yamada, Y.
    Purification and characterization of (S)-tetrahydroberberine oxidase from cultured coptis japonica cells (1988), Phytochemistry, 27, 979-982.
No PubMed abstract available
Search for more literature for 1.3.3.8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Coptis japonica

General Stability

General Stability Organism
instable Coptis japonica

Inhibitors

Inhibitors Comment Organism Structure
bathophenanthroline sulfonic acid specific Fe2+ ion chelator, more than 1 mM required for 50% inhibition at an enzyme concentration of 0.0003 mM Coptis japonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
 (S)-tetrahydroberberine
-
 Coptis japonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, SDS-PAGE Coptis japonica
58000
-
 gel filtration Coptis japonica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-tetrahydroberberine + 2 O2 Coptis japonica final step in berberine biosynthesis berberine + 2 H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Coptis japonica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion-exchange, gel filtration Coptis japonica

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Coptis japonica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 8.1 nkat/mg protein Coptis japonica

Storage Stability

Storage Stability Organism
4°C, 50% loss of activity after 20 h Coptis japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-tetrahydroberberine + 2 O2 four hydrogen atoms from the substrate are transferred to two molecules of oxygen to produce two molecules of hydrogen peroxide and one molecule of berberine Coptis japonica berberine + 2 H2O2
-
 ?
(S)-tetrahydroberberine + 2 O2 final step in berberine biosynthesis Coptis japonica berberine + 2 H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Coptis japonica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Coptis japonica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 50% loss of activity after 20 h Coptis japonica
45
-
 1 h, 40% loss of activity Coptis japonica
50
-
 1 h, 90% loss of activity Coptis japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 50 mM phosphate buffer and borate buffer Coptis japonica