Application | Comment | Organism |
---|---|---|
medicine | ACOX1 is a rate-limiting enzyme in peroxisomal fatty acids beta-oxidation and its deficiency is associated with a lethal, autosomal recessive disease, called pseudoneonatal-adrenoleukodystrophy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strains BL21 and C41, and in COS-7 cells (His-tagged enzyme) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
palmitoyl-CoA | isoform ACOX1a | Homo sapiens | |
0.09 | - |
palmitoyl-CoA | isoform ACOX1b | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
isoform ACOX1a and ACOX1b, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
isoform ACOX1a exhibits only 50% specific activity toward palmitoyl-CoA as compared to ACOX1b | Homo sapiens |
additional information | - |
specific activities in units/mg for isoform ACOX1a: 0.186 for eicosapentaenoyl-CoA, 0.237 for 4-methyl-nonanoyl-CoA, 0.15 for 16-hydroxy-palmitoyl-CoA, 0.336 for 4,8,12-trimethyl-tridecanoyl-CoA, 0.372 for 1,16-hexadecadioyl-CoA, 0.699 for 6-phenyl-6-phenyl-hexanoyl-CoA, 0.076 for palmitoyl-CoA, specific activities in units/mg for isoform ACOX1b: 0.226 for eicosapentaenoyl-CoA, 0.278 for 4-methyl-nonanoyl-CoA, 0.298 for 16-hydroxy-palmitoyl-CoA, 0.423 for 4,8,12-trimethyl-tridecanoyl-CoA, 0.504 for 1,16-hexadecadioyl-CoA, 0.573 for 6-phenyl-6-phenyl-hexanoyl-CoA, 0.166 for palmitoyl-CoA | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,16-hexadecadioyl-CoA + O2 | - |
Homo sapiens | ? | - |
? | |
16-hydroxy-palmitoyl-CoA + O2 | - |
Homo sapiens | ? | - |
? | |
4,8,12-trimethyl-tridecanoyl-CoA + O2 | - |
Homo sapiens | ? | - |
? | |
4-methyl-nonanoyl-CoA + O2 | - |
Homo sapiens | ? | - |
? | |
6-phenyl-6-phenyl-hexanoyl-CoA + O2 | - |
Homo sapiens | ? | - |
? | |
acyl-CoA + O2 | - |
Homo sapiens | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? | |
eicosapentaenoyl-CoA + O2 | - |
Homo sapiens | ? | - |
? | |
palmitoyl-CoA + O2 | - |
Homo sapiens | 2-trans-hexadecenoyl-CoA + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACOX1a | isoform | Homo sapiens |
ACOX1b | isoform | Homo sapiens |
acyl-CoA oxidase 1a | isoform | Homo sapiens |
acyl-CoA oxidase 1b | isoform | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37.5 | - |
the ACOX1b isoform has a maximum specific activity around 37.5°C | Homo sapiens |
40 | - |
the maximum specific activity for ACOX1a is obtained at 40°C | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37.5 | 70 | the ACOX1b isoform retains 57% of its specific activity at 50°C and is more resistant to heat denaturation than ACOX1a since it conserves 30% of its specific activity after treatment at 50°C, the isoform shows 70% specific activity at 37.5°C | Homo sapiens |
50 | - |
the specific activity of isoform ACOX1a drops to zero at 50°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | optimal palmitoyl-CoA oxidase activity for ACOX1b | Homo sapiens |
8.5 | - |
optimal palmitoyl-CoA oxidase activity for ACOX1a | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens |