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Literature summary for 1.3.3.6 extracted from

  • Coudron, P.E.; Frerman, F.E.; Schowalter, D.B.
    Chemical and catalytic properties of the peroxisomal acyl-coenzyme A oxidase from Candida tropicalis (1983), Arch. Biochem. Biophys., 226, 324-336.
    View publication on PubMed
Search for more literature for 1.3.3.6

Inhibitors

Inhibitors Comment Organism Structure
3-octynoyl-CoA irreversible Candida tropicalis
5,5'-dithiobis(2-nitro-benzoic acid) inactivates by modification of sulfhydryl groups and loss of FAD Candida tropicalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 dodecanoyl-CoA pH 7.0-9.5 Candida tropicalis
0.071
-
 dodecanoyl-CoA pH 6.2 Candida tropicalis

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Candida tropicalis 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72100
-
 8 * 72100, SDS-PAGE Candida tropicalis
552000
-
 ultracentrifugation Candida tropicalis

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
 pK 233, inducible by growth on alkanes
-

Purification (Commentary)

Purification (Comment) Organism
-
 Candida tropicalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58
-
 purified enzyme Candida tropicalis

Storage Stability

Storage Stability Organism
-20°C, 25% glycerol, 8 months Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + O2
-
 Candida tropicalis trans-2,3-dehydroacyl-CoA + H2O2
-
 ?
dodecanoyl-CoA + O2
-
 Candida tropicalis (2E)-dodec-2-enoyl-CoA + H2O2
-
 ?
lauroyl-CoA + O2
-
 Candida tropicalis trans-2-dodecenoyl-CoA + H2O2
-
 ?
palmitoyl-CoA + O2
-
 Candida tropicalis 2-trans-hexadecenoyl-CoA + H2O2
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 72100, SDS-PAGE Candida tropicalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information pH-dependency of turnover number Candida tropicalis

Cofactor

Cofactor Comment Organism Structure
FAD tightly bound FAD, 4 mol per mol of enzyme octamer Candida tropicalis