Literature summary for 1.3.3.5 extracted from

Kataoka, K.; Tsukamoto, K.; Kitagawa, R.; Ito, T.; Sakurai, T.
Compensatory binding of an asparagine residue to the coordination-unsaturated type I Cu center in bilirubin oxidase mutants (2008), Biochem. Biophys. Res. Commun., 371, 416-419.

Search for more literature for 1.3.3.5

Cloned(Commentary)

Cloned (Comment)	Organism
-	Albifimbria verrucaria

Protein Variants

Protein Variants	Comment	Organism
M467F	the mutated type I Cu center shows characteristics of phytocyanins	Albifimbria verrucaria
M467L	the mutated type I Cu center shows characteristics of phytocyanins	Albifimbria verrucaria
Met467Q	reduced activity	Albifimbria verrucaria
N459A/M467F	activity is decreased to 1% of the recombinant wild type enzyme, the mutated type I Cu center shows characteristics of phytocyanins, blue copper proteins with an axial coordination of Gln, due to compensatory binding of the distal Asn459	Albifimbria verrucaria

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	contains a type I copper center	Albifimbria verrucaria

Organism

Organism	UniProt	Comment	Textmining
Albifimbria verrucaria	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00041	-	mutant enzyme M467L, using bilirubin as a substrate	Albifimbria verrucaria
0.00047	-	mutant enzyme N459A/M467F, using bilirubin as a substrate	Albifimbria verrucaria
0.00064	-	mutant enzyme M467F, using bilirubin as a substrate	Albifimbria verrucaria
1.19	-	mutant enzyme M467Q, using bilirubin as a substrate	Albifimbria verrucaria
46.1	-	recombinant wild type enzyme, using bilirubin as a substrate	Albifimbria verrucaria

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2	-	Albifimbria verrucaria	?	-	?
bilirubin + O2	-	Albifimbria verrucaria	biliverdin + H2O	-	?
hexacyanoferrate(II) + O2	-	Albifimbria verrucaria	hexacyanoferrate(III) + H2O	-	?
octacyanotungstate(IV) + O2	-	Albifimbria verrucaria	octacyanotungstate(V) + H2O	-	?
p-phenylenediamine + O2	-	Albifimbria verrucaria	?	-	?

Synonyms

Synonyms	Comment	Organism
MvBO	-	Albifimbria verrucaria

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Release 2023.1 (January 2023)