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Literature summary for 1.3.3.5 extracted from
- Point mutations at the type I Cu ligands, Cys457 and Met467, and at the putative proton donor, Asp105, in Myrothecium verrucaria bilirubin oxidase and reactions with dioxygen (2005), Biochemistry, 44, 7004-7012.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Pichia pastoris GS115 transformed using the pPICBO derivative linearized with Bpu1102I | Albifimbria verrucaria |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C457S | can react with dioxygen, affords reaction intermediate I with absorption maxima at 340, 470, and 675 nm | Albifimbria verrucaria |
| D105A | exhibits 7.5% bilirubin oxidase activity compared to the wild-type enzyme, indicating that Asp105 conserved in all multi-copper oxidases donates a proton to reaction intermediates I and II | Albifimbria verrucaria |
| D105E | exhibits 46% bilirubin oxidase activity compared to the wild-type enzyme, indicating that Asp105 conserved in all multi-copper oxidases donates a proton to reaction intermediates I and II | Albifimbria verrucaria |
| D105N | does not react with dioxygen | Albifimbria verrucaria |
| M467G | with modified spectroscopic properties and redox potential, affords reaction intermediate II with absorption maxima at 355 and 450 nm | Albifimbria verrucaria |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
all mutants expressed in Pichia pastoris, SDS-PAGE | Albifimbria verrucaria |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Albifimbria verrucaria | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration | Albifimbria verrucaria |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
no bilirubin oxidase activity of mutant C457S and mutant D105N | Albifimbria verrucaria |
| 0.3 | - |
ferrocyanide oxidase activity of mutant M467G | Albifimbria verrucaria |
| 1.2 | - |
bilirubin oxidase activity of mutant D105A | Albifimbria verrucaria |
| 11 | - |
bilirubin oxidase activity of mutant D105E | Albifimbria verrucaria |
| 24 | - |
bilirubin oxidase activity of authentic enzyme | Albifimbria verrucaria |
| 24 | - |
bilirubin oxidase activity of recombinant enzyme | Albifimbria verrucaria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bilirubin + O2 | - |
Albifimbria verrucaria | biliverdin + H2O | - |
? |  |
| ferrocyanide + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
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Release 2023.1 (January 2023)
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