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Literature summary for 1.3.3.5 extracted from
- High-level expression of Myrothecium verrucaria bilirubin oxidase in Pichia pastoris, and its facile purification and characterization (2005), Protein Expr. Purif., 41, 77-83.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris. The cDNA encoding bilirubin oxidase is cloned into the Pichia pastoris expression vector pPIC9K under the control of the alcohol oxidase 1 promoter and its protein product is secreted using the Saccharomyces alpha-mating factor signal sequence | Albifimbria verrucaria |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
bilirubin | pH 8.0, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria |  |
| 0.12 | - |
bilirubin | pH 8.0, authentic bilirubin oxidase | Albifimbria verrucaria | |
| 0.14 | - |
ditaurobilirubin | pH 5.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
| 0.25 | - |
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) | pH 6.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
| 0.34 | - |
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) | pH 6.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
| 0.37 | - |
ditaurobilirubin | pH 5.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
| 9.6 | - |
p-phenylenediamine | pH 6.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
| 9.8 | - |
p-phenylenediamine | pH 6.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | multicopper oxidase contains 4 copper ions per protein molecule. Cu-binding sites are not affected by differences in carbohydrate content and the N-terminal extension of four amino acid residues | Albifimbria verrucaria | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, SDS-PAGE | Albifimbria verrucaria |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Albifimbria verrucaria | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the recombinant enzyme expressed in Pichia pastoris contains non-native type N-linked sugar chains | Albifimbria verrucaria |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Albifimbria verrucaria |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 57.2 | - |
- |
Albifimbria verrucaria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-dihydroxynaphthalene + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
| 1,5-dihydroxynaphthalene + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
| 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonate) + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
| bilirubin + O2 | - |
Albifimbria verrucaria | biliverdin + H2O | - |
? | |
| ditaurobilirubin + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
| N,N-dimethyl-p-phenylenediamine + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
| o-aminophenol + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
| p-phenylenediamine + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Albifimbria verrucaria |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Albifimbria verrucaria |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life of the recombinant enzyme expressed in Escherichia coli is 90 min, half-life of authentic enzyme is 15 min | Albifimbria verrucaria |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 60 | - |
p-phenylenediamine | pH 6.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
| 90 | - |
p-phenylenediamine | pH 6.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
| 94.3 | - |
ditaurobilirubin | pH 5.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
| 115 | - |
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) | pH 6.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
| 117 | - |
bilirubin | pH 8.0, authentic bilirubin oxidase | Albifimbria verrucaria | |
| 164 | - |
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) | pH 6.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
| 200 | - |
bilirubin | pH 8.0, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
| 233 | - |
ditaurobilirubin | pH 5.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Albifimbria verrucaria |
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