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Literature summary for 1.3.3.4 extracted from

  • Yoon, J.; Han, Y.; Ahn, Y.O.; Hong, M.K.; Sung, S.K.
    Characterization of HemY-type protoporphyrinogen IX oxidase genes from cyanobacteria and their functioning in transgenic Arabidopsis (2019), Plant Mol. Biol., 101, 561-574 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BT3 Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
saflufenacil
-
Arabidopsis thaliana
tiafenacil
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
protoporphyrinogen IX pH and temperature not specified in the publication Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrinogen IX + 3 O2 Arabidopsis thaliana
-
protoporphyrin IX + 3 H2O2
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P55826 ecotype Col-0
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrinogen IX + 3 O2
-
Arabidopsis thaliana protoporphyrin IX + 3 H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Arabidopsis thaliana
monomer 1 * 53100, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
hemY
-
Arabidopsis thaliana
PPO1
-
Arabidopsis thaliana
protoporphyrinogen IX oxidase
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.117
-
protoporphyrinogen IX pH and temperature not specified in the publication Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD
-
Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000293
-
pH and temperature not specified in the publication Arabidopsis thaliana tiafenacil
0.0000373
-
pH and temperature not specified in the publication Arabidopsis thaliana saflufenacil

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.2
-
protoporphyrinogen IX pH and temperature not specified in the publication Arabidopsis thaliana