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Literature summary for 1.3.3.4 extracted from

  • Wang, B.; Zhang, Z.; Zhu, H.; Niu, C.; Wen, X.; Xi, Z.
    The hydrogen bonding network involved Arg59 in human protoporphyrinogen IX oxidase is essential for enzyme activity (2021), Biochem. Biophys. Res. Commun., 557, 20-25 .
    View publication on PubMed
Search for more literature for 1.3.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D349A the mutant enzyme maintains 8.05% of wild type activity Homo sapiens
D349E the mutant enzyme maintains 84.78% of wild type activity Homo sapiens
D349R the mutant enzyme maintains 2.48% of wild type activity Homo sapiens
R59A the mutant enzyme maintains 44.13% of wild type activity Homo sapiens
R59E the mutant enzyme maintains 22.72% of wild type activity Homo sapiens
R59K the mutant enzyme maintains 82.46% of wild type activity Homo sapiens
R59W the mutation dramatically decreases the activity of the enzyme Homo sapiens
T366A the mutant enzyme maintains 65.45% of wild type activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001162
-
 protoporphyrinogen IX mutant enzyme R59A, at pH 7.4 and 25°C Homo sapiens
0.001253
-
 protoporphyrinogen IX mutant enzyme D349E, at pH 7.4 and 25°C Homo sapiens
0.001405
-
 protoporphyrinogen IX wild type enzyme, at pH 7.4 and 25°C Homo sapiens
0.001768
-
 protoporphyrinogen IX mutant enzyme T366A, at pH 7.4 and 25°C Homo sapiens
0.001865
-
 protoporphyrinogen IX mutant enzyme R59K, at pH 7.4 and 25°C Homo sapiens
0.00208
-
 protoporphyrinogen IX mutant enzyme D349A, at pH 7.4 and 25°C Homo sapiens
0.00298
-
 protoporphyrinogen IX mutant enzyme R59E, at pH 7.4 and 25°C Homo sapiens
0.00358
-
 protoporphyrinogen IX mutant enzyme D349R, at pH 7.4 and 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrinogen IX + 3 O2 Homo sapiens
-
 protoporphyrin IX + 3 H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrinogen IX + 3 O2
-
 Homo sapiens protoporphyrin IX + 3 H2O2
-
 ?

Synonyms

Synonyms Comment Organism
PPO
-
 Homo sapiens
protoporphyrinogen IX oxidase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 protoporphyrinogen IX mutant enzyme D349R, at pH 7.4 and 25°C Homo sapiens
0.023
-
 protoporphyrinogen IX mutant enzyme D349A, at pH 7.4 and 25°C Homo sapiens
0.07
-
 protoporphyrinogen IX mutant enzyme R59A, at pH 7.4 and 25°C Homo sapiens
0.092
-
 protoporphyrinogen IX mutant enzyme R59E, at pH 7.4 and 25°C Homo sapiens
0.145
-
 protoporphyrinogen IX mutant enzyme D349E, at pH 7.4 and 25°C Homo sapiens
0.155
-
 protoporphyrinogen IX mutant enzyme T366A, at pH 7.4 and 25°C Homo sapiens
0.192
-
 protoporphyrinogen IX wild type enzyme, at pH 7.4 and 25°C Homo sapiens
0.21
-
 protoporphyrinogen IX mutant enzyme R59K, at pH 7.4 and 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4
-
 protoporphyrinogen IX mutant enzyme D349R, at pH 7.4 and 25°C Homo sapiens
11
-
 protoporphyrinogen IX mutant enzyme D349A, at pH 7.4 and 25°C Homo sapiens
31
-
 protoporphyrinogen IX mutant enzyme R59E, at pH 7.4 and 25°C Homo sapiens
60.2
-
 protoporphyrinogen IX mutant enzyme R59A, at pH 7.4 and 25°C Homo sapiens
87.9
-
 protoporphyrinogen IX mutant enzyme T366A, at pH 7.4 and 25°C Homo sapiens
112.5
-
 protoporphyrinogen IX mutant enzyme R59K, at pH 7.4 and 25°C Homo sapiens
115.7
-
 protoporphyrinogen IX mutant enzyme D349E, at pH 7.4 and 25°C Homo sapiens
136.4
-
 protoporphyrinogen IX wild type enzyme, at pH 7.4 and 25°C Homo sapiens