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Literature summary for 1.3.3.4 extracted from

  • de Pater, S.; Pinas, J.E.; Hooykaas, P.J.; van der Zaal, B.J.
    ZFN-mediated gene targeting of the Arabidopsis protoporphyrinogen oxidase gene through Agrobacterium-mediated floral dip transformation (2013), Plant Biotechnol. J., 11, 510-515 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene PPO, structures of the wild-type genomic PPO locus, a T-DNA repair gene-targeting construct, and the targeted PPO locus, overview Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information design of zinc finger nucleases (ZNFs)-mediated for induction of double-strand breaks at the intended recombination site enhancing the frequency of natural protoporphyrinogen oxidase (PPO) gene targeting at an artificial target locus using Agrobacterium tumefaciens-mediated floral dip transformation. The natural protoporphyrinogen oxidase gene can be conveniently utilized for gene targeting experiments. Wild-type Arabidopsis thaliana plants and plants expressing the ZFNs are transformed via floral dip transformation with a repair T-DNA with an incomplete PPO gene, missing the 5' coding region but containing two mutations rendering the enzyme insensitive to the herbicide butafenacil as well as an extra KpnI site for molecular analysis of gene targeting events. Selection on butafenacil. Analysis of repairing events, overview. One plant line contains a PPO gene repaired only at the 5' end via homologous recombination. Most plant lines contain extra randomly integrated T-DNA copies. Two plant lines do not contain extra T-DNAs, and the repaired PPO genes in these lines are transmitted to the next generation in a Mendelian fashion. Analysis of progeny gene-targeting plants Arabidopsis thaliana
S305L the amino acid exchange causes an insensitivity for the herbicide butafenacil and a KpnI site for detection of gene targeting events Arabidopsis thaliana
Y426M the amino acid exchange causes an insensitivity for the herbicide butafenacil and a KpnI site for detection of gene targeting events Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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Synonyms

Synonyms Comment Organism
PPO
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Arabidopsis thaliana