Application | Comment | Organism |
---|---|---|
additional information | in rice plants, outstanding resistance to Protox-inhibiting herbicides can be achieved by expression of Myxococcus xanthus Protox | Myxococcus xanthus |
additional information | in rice plants, outstanding resistance to Protox-inhibiting herbicides can be achieved by expression of Myxococcus xanthus Protox | synthetic construct |
Cloned (Comment) | Organism |
---|---|
cloned into plasmid pET28(b), expression in Escherichia coli BL21(DE3), subcloned into pGA1611 under the control of constitutive maize ubiquitin promoter and expressed in transgenic rice plant lines S11 and S19 | synthetic construct |
expression in transgenic rice plant lines M4 and and M7 | Myxococcus xanthus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a synthetic Myxococcus xanthus Protox gene, with a sequence optimized to more closely match the codon usage and G+C content of the Arabidopsis Protox gene, decreased G+C content to 49% similar to that of Arapbidopsis | synthetic construct |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxyfuorfen | all wild-type and transgenic rice lines have a significant decrease in Protox activity, all transgenic rice lines expressing native Protox show enhanced resistance to oxyfluorfen as compared to nontransgenic rice | Myxococcus xanthus | |
oxyfuorfen | all wild-type and transgenic rice lines have a significant decrease in Protox activity, all transgenic rice lines expressing modified Protox show enhanced resistance to oxyfluorfen as compared to nontransgenic rice | synthetic construct |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Myxococcus xanthus | 9507 | - |
chloroplast | - |
synthetic construct | 9507 | - |
mitochondrion | - |
Myxococcus xanthus | 5739 | - |
mitochondrion | - |
synthetic construct | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myxococcus xanthus | P56601 | - |
- |
synthetic construct | AY916795 | - |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography | synthetic construct |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer, pH 7.0, 50% glycerol | Myxococcus xanthus |
-20°C, TrisHCl buffer, pH 7.0, 50% glycerol | synthetic construct |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen-IX + 3 O2 | - |
Myxococcus xanthus | protoporphyrin-IX + 3 H2O2 | - |
? | |
protoporphyrinogen-IX + 3 O2 | - |
synthetic construct | protoporphyrin-IX + 3 H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protox | - |
Myxococcus xanthus |
protox | - |
synthetic construct |