Application | Comment | Organism |
---|---|---|
agriculture | enzyme is a target for light-dependent peroxidizing herbicides | Nicotiana tabacum |
Cloned (Comment) | Organism |
---|---|
expression of the thioredoxin- and His-tagged enzyme in Escherichia coli strain B834(DE3), selenomethionine labeling in the auxothrophic strain | Nicotiana tabacum |
Crystallization (Comment) | Organism |
---|---|
purified selenomethionine-labeled, recombinant mitochondrial isozyme PPO2 free or in complex with inhibitor 4-bromo-3-(5'-carboxy-4'-chloro-2'-fluoro-phenyl)-1-methyl-5-trifluoromethyl-pyrazol and with FAD, protein solution contains protein, 5 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, 50 mM sodium chloride, hanging drop vapour diffusion method, 18°C, precipitant solution contains 10% PEG 1000, 0.1 M sodium citrate-HCl, pH 4.0, and 0.2 M sodium chloride, 2 days, selenium single anomalous X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular modelling | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-bromo-3-(5'-carboxy-4'-chloro-2'-fluoro-phenyl)-1-methyl-5-trifluoromethyl-pyrazol | - |
Nicotiana tabacum | |
acifluoren | light-dependent peroxidizing diphenyl ether herbicide | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme PPO1, thylakoid and envelope membranes | Nicotiana tabacum | 9507 | - |
membrane | isozymes PPO1 and PPO2, binding domain and structure | Nicotiana tabacum | 16020 | - |
mitochondrion | isozyme PPO2, outer surface of the inner membrane | Nicotiana tabacum | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, crystal structure | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nicotiana tabacum | the enzyme might be complexed in vivo with the ferrochelatase for substrate channeling | ? | - |
? | |
protoporphyrinogen-IX + 3 O2 | Nicotiana tabacum | enzyme deficiency causes variegate porphyria, bringing about e.g. light-sensitivity of human skin | protoporphyrin-IX + 3 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | O24164 | isozyme PPO1 and PPO2 | - |
Purification (Comment) | Organism |
---|---|
recombinant thioredoxin- and His-tagged, selenomethionine-labeled enzyme from Escherichia coli by nickel affinity chromatography, 3-step purification process | Nicotiana tabacum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2 | reaction mechanism and active site structure | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme might be complexed in vivo with the ferrochelatase for substrate channeling | Nicotiana tabacum | ? | - |
? | |
protoporphyrinogen-IX + 3 O2 | - |
Nicotiana tabacum | protoporphyrin-IX + 3 H2O2 | - |
? | |
protoporphyrinogen-IX + 3 O2 | enzyme deficiency causes variegate porphyria, bringing about e.g. light-sensitivity of human skin | Nicotiana tabacum | protoporphyrin-IX + 3 H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, crystal structure | Nicotiana tabacum |
More | dimer interface structure, domain structure, overview | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
PPO | - |
Nicotiana tabacum |
protoporphyrinogen IX oxidase | - |
Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Nicotiana tabacum |