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Literature summary for 1.3.3.4 extracted from

  • Koch, M.; Breithaupt, C.; Kiefersauer, R.; Freigang, J.; Huber, R.; Messerschmidt, A.
    Crystal structure of protoporphyrinogen IX oxidase: a key enzyme in haem and chlorophyll biosynthesis (2004), EMBO J., 23, 1720-1728.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
agriculture enzyme is a target for light-dependent peroxidizing herbicides Nicotiana tabacum

Cloned(Commentary)

Cloned (Comment) Organism
expression of the thioredoxin- and His-tagged enzyme in Escherichia coli strain B834(DE3), selenomethionine labeling in the auxothrophic strain Nicotiana tabacum

Crystallization (Commentary)

Crystallization (Comment) Organism
purified selenomethionine-labeled, recombinant mitochondrial isozyme PPO2 free or in complex with inhibitor 4-bromo-3-(5'-carboxy-4'-chloro-2'-fluoro-phenyl)-1-methyl-5-trifluoromethyl-pyrazol and with FAD, protein solution contains protein, 5 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, 50 mM sodium chloride, hanging drop vapour diffusion method, 18°C, precipitant solution contains 10% PEG 1000, 0.1 M sodium citrate-HCl, pH 4.0, and 0.2 M sodium chloride, 2 days, selenium single anomalous X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular modelling Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
4-bromo-3-(5'-carboxy-4'-chloro-2'-fluoro-phenyl)-1-methyl-5-trifluoromethyl-pyrazol
-
Nicotiana tabacum
acifluoren light-dependent peroxidizing diphenyl ether herbicide Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isozyme PPO1, thylakoid and envelope membranes Nicotiana tabacum 9507
-
membrane isozymes PPO1 and PPO2, binding domain and structure Nicotiana tabacum 16020
-
mitochondrion isozyme PPO2, outer surface of the inner membrane Nicotiana tabacum 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
2 * 55000, crystal structure Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Nicotiana tabacum the enzyme might be complexed in vivo with the ferrochelatase for substrate channeling ?
-
?
protoporphyrinogen-IX + 3 O2 Nicotiana tabacum enzyme deficiency causes variegate porphyria, bringing about e.g. light-sensitivity of human skin protoporphyrin-IX + 3 H2O2
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum O24164 isozyme PPO1 and PPO2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant thioredoxin- and His-tagged, selenomethionine-labeled enzyme from Escherichia coli by nickel affinity chromatography, 3-step purification process Nicotiana tabacum

Reaction

Reaction Comment Organism Reaction ID
protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2 reaction mechanism and active site structure Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme might be complexed in vivo with the ferrochelatase for substrate channeling Nicotiana tabacum ?
-
?
protoporphyrinogen-IX + 3 O2
-
Nicotiana tabacum protoporphyrin-IX + 3 H2O2
-
?
protoporphyrinogen-IX + 3 O2 enzyme deficiency causes variegate porphyria, bringing about e.g. light-sensitivity of human skin Nicotiana tabacum protoporphyrin-IX + 3 H2O2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 55000, crystal structure Nicotiana tabacum
More dimer interface structure, domain structure, overview Nicotiana tabacum

Synonyms

Synonyms Comment Organism
PPO
-
Nicotiana tabacum
protoporphyrinogen IX oxidase
-
Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Nicotiana tabacum