Any feedback?
Literature summary for 1.3.3.4 extracted from
- Characteristics of human protoporphyrinogen oxidase in controls and variegate porphyrias (1997), Cell. Mol. Biol., 43, 67-73.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in Escherichia coli, His-tag inserted for purification | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A433P | reduced activity | Homo sapiens |
| R168C | reduced activity | Homo sapiens |
| R59W | reducued activity, lacks FAD-cofactor | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
protoporphyrinogen-IX | - |
Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
calculated from amino acid sequence | Homo sapiens |
| 100000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrinogen-IX + 3 O2 | Homo sapiens | penultimate step of heme and chlorophyll biosynthesis | protoporphyrin-IX + 3 H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cloned enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for days, slow proteolysis | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mesoporphyrinogen-IX + 3 O2 | 100times lower activity | Homo sapiens | mesoporphyrin-IX + 3 H2O2 | - |
? | |
| protoporphyrinogen-IX + 3 O2 | - |
Homo sapiens | protoporphyrin-IX + 3 H2O2 | - |
? | |
| protoporphyrinogen-IX + 3 O2 | penultimate step of heme and chlorophyll biosynthesis | Homo sapiens | protoporphyrin-IX + 3 H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 51000 calculated MW, gel filtration | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.175 | - |
protoporphyrinogen-IX | - |
Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | one noncovalently bound FAD per dimer | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
