General Stability | Organism |
---|---|
when incubated with 3 M urea, the enzyme is slowly denatured at pH 6.3 and much faster at pH 7.6 | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
coproporphyrin III | competitive inhibitor | Saccharomyces cerevisiae | |
N-bromosuccinimide | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
70000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + O2 | Saccharomyces cerevisiae | - |
coproporphyrin III + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P11353 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Ultrogel Ac44 gel filtration | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00925 | - |
unpurified enzyme, at pH 7.6 and 25°C | Saccharomyces cerevisiae |
0.08483 | - |
purified enzyme, at pH 7.6 and 25°C | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 1% (w/v) NaN3 (100 mM Tris, pH 7.6), several years, no loss of activity | Saccharomyces cerevisiae |
25°C, 1% (w/v) NaN3 (100 mM Tris, pH 7.6), several months, no loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + O2 | - |
Saccharomyces cerevisiae | coproporphyrin III + H2O | - |
? | |
additional information | no anaerobic activity is detected using NADP+ and S-adenosylmethionine | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
coproporphyrinogen III oxidase | - |
Saccharomyces cerevisiae |
CPO | - |
Saccharomyces cerevisiae |
HEM13 | - |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
a 2 minute incubation of the protein at 56°C leads to a total loss of enzymatic activity | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | isoelectric focusing | - |
6.2 |