Literature summary for 1.3.3.3 extracted from

Gitter, S.J.; Cooper, C.L.; Friesen, J.A.; Jones, M.A.
Investigation of the catalytic and structural roles of conserved histidines of human coproporphyrinogen oxidase using site-directed mutagenesis (2006), Med. Sci. Monit., 13, BR1-BR10.

Search for more literature for 1.3.3.3

Application

Application	Comment	Organism
medicine	His158 may have a role in the active site of CPO, none of the conserved histidine residues of the enzyme are essential for catalytic activity although changes in histidines are implicated in the disease state hereditary coproporphyria	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21(DE3)RIL	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
H148A	retains 39% of wild type enzyme activity for the overall conversion of coproporphyrinogen-III to protoporphyrinogen-IX	Homo sapiens
H158A	the mutant exhibits approximately 50fold lower activity than wild type recombinant CPO for the conversion of coproporphyrinogen-III to protoporphyrinogen-IX	Homo sapiens
H197A	the mutant exhibits approximately 50fold lower activity for the overall conversion of coproporphyrinogen-III to protoporphyrinogen-IX than wild type recombinant CPO, but the second oxidative decarboxylation step is not impaired, with mutant enzyme H197A retaining 100% of the wild type activity using harderoporphyrinogen as substrate	Homo sapiens
H227A	catalyzes the conversion of coproporphyrinogen-III to protoporphyrinogen-IX at a rate almost 2fold that of the wild type enzyme	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00015	-	Coproporphyrinogen-III	mutant enzyme H158A, at 37°C	Homo sapiens
0.00015	-	Harderoporphyrinogen	mutant enzyme H158A, at 37°C	Homo sapiens
0.0003	-	Coproporphyrinogen-III	wild type enzyme, at 37°C	Homo sapiens
0.0012	-	Harderoporphyrinogen	wild type enzyme, at 37°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P36551	precursor	-

Purification (Commentary)

Purification (Comment)	Organism
TALON metal affinity resin column chromatography	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0017	-	wild type enzyme, for mesoporphyrinogen-VI, at 37°C	Homo sapiens
0.0049	-	wild type enzyme, for coproporphyrinogen-III, at 37°C	Homo sapiens
0.0051	-	wild type enzyme, for harderoporphyrinogen, at 37°C	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
coproporphyrinogen-III + O2 + 2 H+	-	Homo sapiens	protoporphyrinogen-IX + 2 CO2 + 2 H2O	-	?
coproporphyrinogen-III + O2 + 2 H+	-	Homo sapiens	harderoporphyrinogen + CO2 + 2 H2O	-	?
harderoporphyrinogen + O2 + 2 H+	-	Homo sapiens	protoporphyrinogen-IX + CO2 + 2 H2O	-	?

Synonyms

Synonyms	Comment	Organism
CPO	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00025	-	Coproporphyrinogen-III	mutant H158A	Homo sapiens
0.000383	-	Harderoporphyrinogen	mutant H158A	Homo sapiens
0.06	-	Coproporphyrinogen-III	wild-type	Homo sapiens
0.1317	-	Harderoporphyrinogen	wild-type	Homo sapiens

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Release 2023.1 (January 2023)