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Literature summary for 1.3.3.3 extracted from

  • Tait, G.H.
    Coproporphyrinogenase activities in extracts of Rhodopseudomonas spheroids and Chromatium strain D (1972), Biochem. J., 128, 1159-1169.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
L-methionine maximal activity at 0.5 mM Cereibacter sphaeroides
S-adenosyl-L-methionine can replace NADP+-NADH in anaerobic reaction Chromatium sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 90% inhibition of anaerobic reaction at 0.17 mM Cereibacter sphaeroides
1,4-Naphthoquinone 61% inhibition at 0.015 mM Cereibacter sphaeroides
2,2-bipyridyl 77% inhibition of anaerobic reaction at 1.5 mM Cereibacter sphaeroides
2,4-dinitrophenol 95% inhibition of anaerobic reaction at 1 mM Cereibacter sphaeroides
FAD 95% inhibition of anaerobic reaction at 0.03 mM Cereibacter sphaeroides
FMN 95% inhibition of anaerobic reaction at 0.03 mM Cereibacter sphaeroides
riboflavin 95% inhibition of anaerobic reaction at 0.03 mM Cereibacter sphaeroides
S-adenosylethionine marked inhibition of anaerobic reaction Cereibacter sphaeroides
S-adenosylhomocysteine marked inhibition of anaerobic reaction Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03 0.05 Coproporphyrinogen-III
-
Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coproporphyrinogen-III + ? Cereibacter sphaeroides anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ protoporphyrinogen-IX + ?
-
?
coproporphyrinogen-III + ? Chromatium sp. anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ protoporphyrinogen-IX + ?
-
?
coproporphyrinogen-III + ? Chromatium sp. D anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ protoporphyrinogen-IX + ?
-
?
coproporphyrinogen-III + O2 + 2 H+ Cereibacter sphaeroides
-
protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
?
coproporphyrinogen-III + O2 + 2 H+ Chromatium sp.
-
protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
?
coproporphyrinogen-III + O2 + 2 H+ Chromatium sp. D
-
protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Chromatium sp.
-
-
-
Chromatium sp. D
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Cereibacter sphaeroides

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Cereibacter sphaeroides
-
cell culture
-
Chromatium sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen-III + ? anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ Cereibacter sphaeroides protoporphyrinogen-IX + ?
-
?
coproporphyrinogen-III + ? anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ Chromatium sp. protoporphyrinogen-IX + ?
-
?
coproporphyrinogen-III + ? anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ Chromatium sp. D protoporphyrinogen-IX + ?
-
?
coproporphyrinogen-III + O2 + 2 H+
-
Cereibacter sphaeroides protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
?
coproporphyrinogen-III + O2 + 2 H+
-
Chromatium sp. protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
?
coproporphyrinogen-III + O2 + 2 H+
-
Chromatium sp. D protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
aerobic activity Cereibacter sphaeroides
8.5
-
anaerobic reaction Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
ATP
-
Chromatium sp.
ATP strictly required for anaerobic enzyme Cereibacter sphaeroides
NADP+ only anaerobic enzyme, maximum activity together with NADH Cereibacter sphaeroides
NADP+ only anaerobic enzyme, maximum activity together with NADH Chromatium sp.