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Literature summary for 1.3.3.15 extracted from

  • Hansson, M.; Hederstedt, L.
    Bacillus subtilis HemY is a peripheral membrane protein essential for protoheme IX synthesis which can oxidize coproporphyrinogen III and protoporphyrinogen IX (1994), J. Bacteriol., 176, 5962-5970.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
protoporphyrinogen IX acts as a competitive inhibitor of the coproporphyrinogen III to coproporphyrin oxidase activity of HemY Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bacillus subtilis 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 53000, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coproporphyrinogen III + O2 Bacillus subtilis 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate coproporphyrin III + H2O
-
 ?
coproporphyrinogen III + O2 Bacillus subtilis 3G18 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate coproporphyrin III + H2O
-
 ?
protoporphyrinogen IX + O2 Bacillus subtilis
-
 protoporphyrin IX + H2O
-
 ?
protoporphyrinogen IX + O2 Bacillus subtilis 3G18
-
 protoporphyrin IX + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 3G18
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen III + O2 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate Bacillus subtilis coproporphyrin III + H2O
-
 ?
coproporphyrinogen III + O2 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate Bacillus subtilis 3G18 coproporphyrin III + H2O
-
 ?
additional information the enzyme does not oxidize uroporphyrinogen III Bacillus subtilis ?
-
 ?
additional information the enzyme does not oxidize uroporphyrinogen III Bacillus subtilis 3G18 ?
-
 ?
protoporphyrinogen IX + O2
-
 Bacillus subtilis protoporphyrin IX + H2O
-
 ?
protoporphyrinogen IX + O2
-
 Bacillus subtilis 3G18 protoporphyrin IX + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Bacillus subtilis
? x * 51203, calculated from amino acid sequence Bacillus subtilis

Synonyms

Synonyms Comment Organism
hemY
-
 Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0003
-
 protoporphyrinogen IX at pH 7.2 and 30°C Bacillus subtilis