General Stability | Organism |
---|---|
no loss of activity is observed on freezing | Streptomyces galilaeus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | 5 mM, 44% inhibition | Streptomyces galilaeus | |
Fe2+ | 0.5 mM, 26% inhibition. 1 mM, 73% inhibition | Streptomyces galilaeus | |
NaN3 | 1 mM, 15% inhibition. 5 mM, 53% inhibition | Streptomyces galilaeus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
gel filtration | Streptomyces galilaeus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
aclacinomycin A + O2 | Streptomyces galilaeus | - |
aclacinomycin Y + H2O2 | - |
? | |
aclacinomycin A + O2 | Streptomyces galilaeus MA144-M1 6U-21 | - |
aclacinomycin Y + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces galilaeus | - |
- |
- |
Streptomyces galilaeus MA144-M1 6U-21 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces galilaeus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
952 | - |
pH and temperature not specified in the publication | Streptomyces galilaeus |
Storage Stability | Organism |
---|---|
-20°C, in 10% glycerol, 0.05 M Tris-HCl, pH 7.2, stable for at least 2 months | Streptomyces galilaeus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aclacinomycin A + O2 | - |
Streptomyces galilaeus | aclacinomycin Y + H2O2 | - |
? | |
aclacinomycin A + O2 | the enzyme catalyzes the oxidation of the terminal sugar, L-cinerulose, of the trisaccharide moiety of aclacinomycin A to L-aculose (2,3,6-trideoxyhex-2-enopyranos-4-ulose) with removal of two electrons. NAD+, NADP+, phenazine methosulfate and 2,6-dichlorophenolindophenol can not serve as electron acceptors | Streptomyces galilaeus | aclacinomycin Y + H2O2 | - |
? | |
aclacinomycin A + O2 | - |
Streptomyces galilaeus MA144-M1 6U-21 | aclacinomycin Y + H2O2 | - |
? | |
aclacinomycin A + O2 | the enzyme catalyzes the oxidation of the terminal sugar, L-cinerulose, of the trisaccharide moiety of aclacinomycin A to L-aculose (2,3,6-trideoxyhex-2-enopyranos-4-ulose) with removal of two electrons. NAD+, NADP+, phenazine methosulfate and 2,6-dichlorophenolindophenol can not serve as electron acceptors | Streptomyces galilaeus MA144-M1 6U-21 | aclacinomycin Y + H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7, 30 min, stable | Streptomyces galilaeus |
60 | - |
pH 7, 30 min, 20% loss of activity | Streptomyces galilaeus |
80 | - |
pH 7, 30 min, 50% loss of activity | Streptomyces galilaeus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Streptomyces galilaeus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.8 | 7 | pH 3.8: about 40% of maximal activity, pH 7.0: about 65% of maximal activity | Streptomyces galilaeus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 37°C, 3 h, stable | Streptomyces galilaeus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces galilaeus | isoelectric focusing | - |
5.9 |