Application | Comment | Organism |
---|---|---|
biotechnology | cofactor engineering of PQQ in Gluconobacter oxydans is beneficial for enhancing the production of quinoprotein-related products | Gluconobacter oxydans |
synthesis | cofactor engineering of PQQ in Gluconobacter oxydans is beneficial for enhancing the production of quinoprotein-related products | Gluconobacter oxydans |
Cloned (Comment) | Organism |
---|---|
gene pqqC, enzymes that biosynthesize PQQ are encoded by the pqqABCDE gene cluster and the tldD gene. The tufB promoter is ligated withpqqA, pqqB, pqqC, pqqD, pqqE and tldD, and the pqqA promoter is ligated with pqqB, pqqC, pqqD and pqqE, respectively, through fusionPCR, subcloning in Escherichia coli. Overexpression Gluconobacter oxydans strain WSH-003 of pqqA, pqqB, pqqC, pqqD and pqqE with the pqqA promoter enhances the extracellular PQQ concentration by 38.5%, 68.4%, 19.9%, 30.3% and 8.2%, respectively, whereas production is enhanced by 59.4%, 85.7%, 30.9%, 42.2%, and 19.3%, respectively, using the tufB promoter. Metabolic effects, overview | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 | Gluconobacter oxydans | - |
4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O | - |
? | |
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 | Gluconobacter oxydans WSH-003 | - |
4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | Q9L3B2 | - |
- |
Gluconobacter oxydans WSH-003 | Q9L3B2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 | - |
Gluconobacter oxydans | 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O | - |
? | |
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 | - |
Gluconobacter oxydans WSH-003 | 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PqqC | - |
Gluconobacter oxydans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in biosynthesis of pyrroloquinoline quinone, PQQ, a cofactor of various membrane-bound dehydrogenases. The amount of endogenous PQQ is generally regarded as a bottleneck to achieving higher catalytic efficiency of PQQ-dependent dehydrogenases. PQQ biosynthesis can be enhanced by overexpressing some of the individual genes in the gene cluster in Gluconobacter oxydans and the PQQ levels are positively correlated with the efficiency of conversion of D-sorbitol to L-sorbose, overexpression of PQQ-related genes activates L-sorbose production and D-sorbitol dehydrogenase activity | Gluconobacter oxydans |