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Literature summary for 1.3.2.3 extracted from

  • Imai, T.; Niwa, M.; Ban, Y.; Hirai, M.; Oba, K.; Moriguchi, T.
    Importance of the L-galactonolactone pool for enhancing the ascorbate content revealed by L-galactonolactone dehydrogenase-overexpressing tobacco plants (2009), Plant Cell Tissue Organ Cult., 96, 105-112.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
cDNA encoding GLDH, subcloning in Escherichia coli strain HB101, and functional expression in transgenic Nicotiana tabacum leaves and roots via Agrobacterium tumefaciens LBA4404-mediated transformation under control of the CaMV 35S promoter. Despite the elevated GLDH activity, the L-ascorbate content in the leaves does not change in all lines, but increases 6-10fold in the roots of transgenic plants, overview Ipomoea batatas

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Ipomoea batatas 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactono-1,4-lactone + cytochrome c Ipomoea batatas
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L-ascorbate + reduced cytochrome c
-
?
additional information Ipomoea batatas incubation of leaf discs in a L-galactonolactone solution leads to a rapid 2 to 3fold increase in the L-ascorbate content in both GLDH-transformed and non-transformed plants in the same manner ?
-
?

Organism

Organism UniProt Comment Textmining
Ipomoea batatas
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Ipomoea batatas
-
root
-
Ipomoea batatas
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0245 0.0513 root mitochondria of transformed transgenic tobacco lines, pH not specified in the publication, temperature not specified in the publication Ipomoea batatas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactono-1,4-lactone + cytochrome c
-
Ipomoea batatas L-ascorbate + reduced cytochrome c
-
?
additional information incubation of leaf discs in a L-galactonolactone solution leads to a rapid 2 to 3fold increase in the L-ascorbate content in both GLDH-transformed and non-transformed plants in the same manner Ipomoea batatas ?
-
?

Synonyms

Synonyms Comment Organism
GalL dehydrogenase
-
Ipomoea batatas
GLDH
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Ipomoea batatas
L-galactono-1,4-lactone dehydrogenase
-
Ipomoea batatas

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
Ipomoea batatas

General Information

General Information Comment Organism
metabolism GLDH catalyzes the last step of L-ascorbate, AsA, biosynthesis in plants, but the upstream genes in the AsA biosynthetic pathway are responsible for enhancing the AsA content in plants, regulation, overview Ipomoea batatas