Cloned (Comment) | Organism |
---|---|
into a pET-His6 vector for expression in Escherichia coli BL21DE3 cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C340A | mutant, insensitive toward thiol oxidation, exhibits poor affinity for L-galactono-1,4-lactone | Arabidopsis thaliana |
C340S | mutant, insensitive toward thiol oxidation, exhibits poor affinity for L-galactono-1,4-lactone | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | thiol-modifying agent | Arabidopsis thaliana | |
H2O2 | GALDH is inactivated by hydrogen peroxide due to selective oxidation of cysteine 340, located in the cap domain | Arabidopsis thaliana | |
N-ethylmaleimide | thiol-modifying agent | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
L-galactono-1,4-lactone | wild-type enzyme | Arabidopsis thaliana | |
5 | - |
L-galactono-1,4-lactone | mutant C340S | Arabidopsis thaliana | |
9 | - |
L-galactono-1,4-lactone | mutant C340A | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + ferricytochrome c | Arabidopsis thaliana | - |
L-ascorbate + ferrocytochrome c + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SU56 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + ferricytochrome c | - |
Arabidopsis thaliana | L-ascorbate + ferrocytochrome c + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
galactonolactone dehydrogenase | - |
Arabidopsis thaliana |
GALDH | - |
Arabidopsis thaliana |
L-galactono-gamma-lactone dehydrogenase | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
59 | - |
L-galactono-1,4-lactone | mutant C340S | Arabidopsis thaliana | |
79 | - |
L-galactono-1,4-lactone | mutant C340A | Arabidopsis thaliana | |
134 | - |
L-galactono-1,4-lactone | wild-type enzyme | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana |