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Literature summary for 1.3.1.98 extracted from

  • Sylvester, D.R.; Alvarez, E.; Patel, A.; Ratnam, K.; Kallender, H.; Wallis, N.G.
    Identification and characterization of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from the gram-positive pathogen Streptococcus pneumoniae (2001), Biochem. J., 355, 431-435.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.98

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of protein in Escherichia coli strain BL21(DE3) Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Streptococcus pneumoniae
Pb2+
-
 Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.079
-
 NADPH
-
 Streptococcus pneumoniae
0.089
-
 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+
-
 Streptococcus pneumoniae
K+ without monovalent cations, only minimium enzyme activity Streptococcus pneumoniae
K+ K+ causes greatest activation Streptococcus pneumoniae
additional information divalent cations have no activating effect Streptococcus pneumoniae
additional information no activation with Li+ Streptococcus pneumoniae
Na+
-
 Streptococcus pneumoniae
NH4+
-
 Streptococcus pneumoniae
Rb+
-
 Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 reducing SDS-PAGE Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Streptococcus pneumoniae
-
 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Streptococcus pneumoniae 100993
-
 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
 strain 0100993
-
Streptococcus pneumoniae 100993
-
 strain 0100993
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus pneumoniae

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetyl-alpha-D-muramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH + H+ ping pong mechanism, contains FAD Streptococcus pneumoniae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
 Streptococcus pneumoniae UDP-N-acetyl-3-D-muramate + NADP+
-
 ?
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
 Streptococcus pneumoniae 100993 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD absorbance spectrum is characteristic of flavoproteins Streptococcus pneumoniae
additional information NADH not substrate Streptococcus pneumoniae
additional information thio-NADH, nicotinamide hyopxanthine dinucleotide in reduced form not substrates Streptococcus pneumoniae
additional information 3'-NADPH, alpha-NADPH are substrates Streptococcus pneumoniae
NADPH
-
 Streptococcus pneumoniae
nicotinamide hypoxanthine dinucleotide phosphate reduced form, active substrate Streptococcus pneumoniae