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Literature summary for 1.3.1.98 extracted from

  • Taku, A.; Gunetileke, K.G.; Anwar, R.A.
    Biosynthesis of uridine diphospho-N-acetylmuramic acid. 3. Purification and properties of uridine diphospho-N-acetylenolpyruvyl-glucosamine reductase (1970), J. Biol. Chem., 245, 5012-5016.
    View publication on PubMed
Search for more literature for 1.3.1.98

Activating Compound

Activating Compound Comment Organism Structure
FAD 11-25% stimulation Enterobacter cloacae

General Stability

General Stability Organism
dithiothreitol stabilizes Enterobacter cloacae

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
 Enterobacter cloacae
N-ethylmaleimide
-
 Enterobacter cloacae
p-chloromercuribenzoate
-
 Enterobacter cloacae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 Enterobacter cloacae
0.049
-
 NADPH
-
 Enterobacter cloacae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Enterobacter cloacae
-
 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Enterobacter cloacae NRC 492
-
 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
 NRC 492
-
Enterobacter cloacae NRC 492
-
 NRC 492
-

Oxidation Stability

Oxidation Stability Organism
very sensitive to oxidation, rapid loss of activity in absence of added dithiothreitol Enterobacter cloacae

Purification (Commentary)

Purification (Comment) Organism
-
 Enterobacter cloacae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
-
 Enterobacter cloacae

Storage Stability

Storage Stability Organism
-20°C, pH 8.0, presence of dithiothreitol, up to 2 months without loss of activity Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
 Enterobacter cloacae UDP-N-acetyl-3-D-muramate + NADP+
-
 ?
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
 Enterobacter cloacae NRC 492 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 increase of activity up to 40°C Enterobacter cloacae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50 40°C: activity maximum, 50°C: about 30% of activity maximum Enterobacter cloacae
50
-
 at 50°C about 30% of maximal activity Enterobacter cloacae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1 8.5 in Tris-HCl buffer maximal activity Enterobacter cloacae

pH Range

pH Minimum pH Maximum Comment Organism
7.1 9.9 at pH 7.1 and pH 9.9 about 60% of maximal activity Enterobacter cloacae

Cofactor

Cofactor Comment Organism Structure
FAD prosthetic group: FAD, Na2S2O4, NaBH4 and to a lesser extent NADH can replace NADPH Enterobacter cloacae
additional information no effect with FMN Enterobacter cloacae
NADH reductase activity with NADH is only 5% of that with NADPH Enterobacter cloacae
NADPH
-
 Enterobacter cloacae