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Literature summary for 1.3.1.97 extracted from

  • Okada, S.; Devarenne, T.P.; Murakami, M.; Abe, H.; Chappell, J.
    Characterization of botryococcene synthase enzyme activity, a squalene synthase-like activity from the green microalga Botryococcus braunii, Race B (2004), Arch. Biochem. Biophys., 422, 110-118.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Botryococcus braunii

Inhibitors

Inhibitors Comment Organism Structure
squalestatin
-
Botryococcus braunii
Triton X-100
-
Botryococcus braunii
Tween 80 enzyme activity is completely abolished by 0.5% (v/v) Tween 80 Botryococcus braunii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Botryococcus braunii 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Botryococcus braunii
additional information no enzyme activity is evident with Co2+ or Mn2+ as a cofactor Botryococcus braunii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52500
-
x * 52500, estimated from amino acid sequence and SDS-PAGE Botryococcus braunii

Organism

Organism UniProt Comment Textmining
Botryococcus braunii
-
race B
-
Botryococcus braunii Berkeley (Showa)
-
race B
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
presqualene diphosphate + NADPH + H+
-
Botryococcus braunii C30 botryococcene + NADP+ + diphosphate
-
?
presqualene diphosphate + NADPH + H+
-
Botryococcus braunii Berkeley (Showa) C30 botryococcene + NADP+ + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 52500, estimated from amino acid sequence and SDS-PAGE Botryococcus braunii

Synonyms

Synonyms Comment Organism
botryococcene synthase
-
Botryococcus braunii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Botryococcus braunii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
in MOPS buffer Botryococcus braunii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Botryococcus braunii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000001
-
squalestatin at pH 7.3 and 37°C Botryococcus braunii

Expression

Organism Comment Expression
Botryococcus braunii gene expression is preferential during rapid growth. Enzyme activity is initially low in the cells, but rises greater than 10fold to a maximum by day 3, and then decreases gradually to almost undetectable levels by the end of the culture period up