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show all sequences of 1.3.1.95

Purification and some properties of an acryloyl-CoA reductase of Clostridium kluyveri

Sedlmeier, H.; Simon, H.; Biol. Chem. Hoppe-Seyler 366, 953-961 (1985)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ethyl acrylate
strong inhibitor of the reduction of ethyl vinyl ketone
Clostridium kluyveri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00083
-
acryloyl-CoA
in 0.1 M phosphate buffer pH 6.4, at 22°C
Clostridium kluyveri
0.008
-
(E)-2-butenoyl-CoA
in 0.1 M phosphate buffer pH 6.4, at 22°C
Clostridium kluyveri
0.77
-
ethyl vinyl ketone
in 0.1 M phosphate buffer pH 6.4, at 22°C
Clostridium kluyveri
5.6
-
acrolein
in 0.1 M phosphate buffer pH 6.4, at 22°C
Clostridium kluyveri
35
-
N-acetyl-S-acryloylcysteamine
in 0.1 M phosphate buffer pH 6.4, at 22°C
Clostridium kluyveri
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
does not contain iron
Clostridium kluyveri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14200
-
2 * 14200, SDS-PAGE
Clostridium kluyveri
28400
-
gel filtration
Clostridium kluyveri
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium kluyveri
-
-
-
Oxidation Stability
Oxidation Stability
Organism
the enzyme exhibits up to 11% and up to 16% residual activity after 19 days of exposure to air at pH 8.0 and 10°C and 30°C, respectively
Clostridium kluyveri
Purification (Commentary)
Commentary
Organism
DEAE-Sepharose CL-6B column chromatography, phenyl-Sepharose CL-4B column chromatography, CM-Sephadex A-25 gel filtration, and Sephacryl S-200 gel filtration
Clostridium kluyveri
Storage Stability
Storage Stability
Organism
-18°C, 30% (w/v) ethylene glycol in 15 mM phosphate buffer pH 8.0, 1 year, 20% loss of activity
Clostridium kluyveri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-2-butenoate + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
(E)-2-butenoyl-CoA + methyl viologen
second best substrate, 45% activity compared to acryloyl-CoA
696519
Clostridium kluyveri
?
-
-
-
?
1-penten-3-one + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
2-cyclohexen-1-one + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
2-methylacrolein + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
4-phenyl-3-buten-2-one + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
acrolein + methyl viologen
-
696519
Clostridium kluyveri
?
-
-
-
?
acrylic acid + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
acryloyl-CoA + methyl viologen
best substrate
696519
Clostridium kluyveri
?
-
-
-
?
butyryl-CoA + 2,6-dichlorophenolindophenol
about 1% of the reduction rate of (E)-2-butenoyl-CoA and methyl viologen
696519
Clostridium kluyveri
?
-
-
-
?
diethyl ketone + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
ethyl acrylate + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
ethyl vinyl ketone + methyl viologen
-
696519
Clostridium kluyveri
?
-
-
-
?
methyl propenyl ketone + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
methyl vinyl ketone + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
additional information
the enzyme shows no activity with different concentrations of NAD(P)H in 0.1 M phosphate buffer pH 7.0 and ethyl vinyl ketone as substrate
696519
Clostridium kluyveri
?
-
-
-
-
N-acetyl-S-acryloylcysteamine + methyl viologen
-
696519
Clostridium kluyveri
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 14200, SDS-PAGE
Clostridium kluyveri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.9
-
two optima at pH 5.9 and 6.4
Clostridium kluyveri
6.4
-
two optima at pH 5.9 and 6.4
Clostridium kluyveri
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.9
8.3
the activity of the acryloyl-CoA reductase decreases to about 30% at pH 3.9 or pH 8.3, respectively
Clostridium kluyveri
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
contains 1.5 mol FMN
Clostridium kluyveri
additional information
he activity of the acryloyl-CoA reductase is not altered significantly by any of the following substances added in concentrations of 0.075 to 0.15 mM: FMN, FAD, AMP, ADP, ATP, NAD+, NADH, acetyl phosphate and co-enzyme A
Clostridium kluyveri
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Clostridium kluyveri
isoelectric focusing
-
7.5
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
contains 1.5 mol FMN
Clostridium kluyveri
additional information
he activity of the acryloyl-CoA reductase is not altered significantly by any of the following substances added in concentrations of 0.075 to 0.15 mM: FMN, FAD, AMP, ADP, ATP, NAD+, NADH, acetyl phosphate and co-enzyme A
Clostridium kluyveri
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ethyl acrylate
strong inhibitor of the reduction of ethyl vinyl ketone
Clostridium kluyveri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00083
-
acryloyl-CoA
in 0.1 M phosphate buffer pH 6.4, at 22°C
Clostridium kluyveri
0.008
-
(E)-2-butenoyl-CoA
in 0.1 M phosphate buffer pH 6.4, at 22°C
Clostridium kluyveri
0.77
-
ethyl vinyl ketone
in 0.1 M phosphate buffer pH 6.4, at 22°C
Clostridium kluyveri
5.6
-
acrolein
in 0.1 M phosphate buffer pH 6.4, at 22°C
Clostridium kluyveri
35
-
N-acetyl-S-acryloylcysteamine
in 0.1 M phosphate buffer pH 6.4, at 22°C
Clostridium kluyveri
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
does not contain iron
Clostridium kluyveri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14200
-
2 * 14200, SDS-PAGE
Clostridium kluyveri
28400
-
gel filtration
Clostridium kluyveri
Oxidation Stability (protein specific)
Oxidation Stability
Organism
the enzyme exhibits up to 11% and up to 16% residual activity after 19 days of exposure to air at pH 8.0 and 10°C and 30°C, respectively
Clostridium kluyveri
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Sepharose CL-6B column chromatography, phenyl-Sepharose CL-4B column chromatography, CM-Sephadex A-25 gel filtration, and Sephacryl S-200 gel filtration
Clostridium kluyveri
Storage Stability (protein specific)
Storage Stability
Organism
-18°C, 30% (w/v) ethylene glycol in 15 mM phosphate buffer pH 8.0, 1 year, 20% loss of activity
Clostridium kluyveri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-2-butenoate + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
(E)-2-butenoyl-CoA + methyl viologen
second best substrate, 45% activity compared to acryloyl-CoA
696519
Clostridium kluyveri
?
-
-
-
?
1-penten-3-one + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
2-cyclohexen-1-one + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
2-methylacrolein + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
4-phenyl-3-buten-2-one + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
acrolein + methyl viologen
-
696519
Clostridium kluyveri
?
-
-
-
?
acrylic acid + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
acryloyl-CoA + methyl viologen
best substrate
696519
Clostridium kluyveri
?
-
-
-
?
butyryl-CoA + 2,6-dichlorophenolindophenol
about 1% of the reduction rate of (E)-2-butenoyl-CoA and methyl viologen
696519
Clostridium kluyveri
?
-
-
-
?
diethyl ketone + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
ethyl acrylate + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
ethyl vinyl ketone + methyl viologen
-
696519
Clostridium kluyveri
?
-
-
-
?
methyl propenyl ketone + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
methyl vinyl ketone + methyl viologen
less than 2% activity of that with ethyl vinyl ketone
696519
Clostridium kluyveri
?
-
-
-
?
additional information
the enzyme shows no activity with different concentrations of NAD(P)H in 0.1 M phosphate buffer pH 7.0 and ethyl vinyl ketone as substrate
696519
Clostridium kluyveri
?
-
-
-
-
N-acetyl-S-acryloylcysteamine + methyl viologen
-
696519
Clostridium kluyveri
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 14200, SDS-PAGE
Clostridium kluyveri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.9
-
two optima at pH 5.9 and 6.4
Clostridium kluyveri
6.4
-
two optima at pH 5.9 and 6.4
Clostridium kluyveri
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.9
8.3
the activity of the acryloyl-CoA reductase decreases to about 30% at pH 3.9 or pH 8.3, respectively
Clostridium kluyveri
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Clostridium kluyveri
isoelectric focusing
-
7.5
Other publictions for EC 1.3.1.95
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718680
Kandasamy
Engineering Escherichia coli w ...
Anaerotignum propionicum, Anaerotignum propionicum DSM 1682
Appl. Microbiol. Biotechnol.
97
1191-1200
2013
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-
1
-
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4
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-
1
-
2
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2
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1
2
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-
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-
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-
1
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
698624
Teufel
3-Hydroxypropionyl-coenzyme A ...
Metallosphaera sedula, Sulfolobus tokodaii
J. Bacteriol.
191
4572-4581
2009
-
-
1
-
-
-
-
4
-
2
4
-
-
8
-
-
2
-
-
-
4
-
3
2
-
-
-
2
1
-
-
4
-
-
-
-
-
1
4
-
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-
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-
4
-
2
4
-
-
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2
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4
-
3
2
-
-
-
2
1
-
-
-
-
-
-
-
-
-
697707
Hetzel
Acryloyl-CoA reductase from Cl ...
Anaerotignum propionicum
Eur. J. Biochem.
270
902-910
2003
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-
-
-
-
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-
3
1
1
3
-
-
5
-
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1
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1
2
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3
1
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-
3
-
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3
-
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-
3
-
-
-
-
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-
3
1
1
3
-
-
-
-
1
-
1
2
-
3
1
-
-
-
3
-
-
-
-
-
-
-
-
3
3
696519
Sedlmeier
Purification and some properti ...
Clostridium kluyveri
Biol. Chem. Hoppe-Seyler
366
953-961
1985
-
-
-
-
-
-
1
5
-
1
2
-
-
2
1
-
1
-
-
-
-
1
17
1
-
-
-
-
2
1
-
2
-
1
-
-
-
-
2
-
-
-
-
1
-
5
-
1
2
-
-
1
-
1
-
-
-
1
17
1
-
-
-
-
2
1
-
1
-
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-
-
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