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Literature summary for 1.3.1.9 extracted from

  • Radka, C.D.; Frank, M.W.; Yao, J.; Seetharaman, J.; Miller, D.J.; Rock, C.O.
    The genome of a Bacteroidetes inhabitant of the human gut encodes a structurally distinct enoyl-acyl carrier protein reductase (FabI) (2020), J. Biol. Chem., 295, 7635-7652 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Alistipes finegoldii

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure at 1.72 A resolution, protein adopts a Rossmann fold. FabI/NADH crystal structure at 1.86 A resolution, the protein undergoes a large conformational change to participate in covering the NADH-binding pocket Alistipes finegoldii

Inhibitors

Inhibitors Comment Organism Structure
triclosan
-
Alistipes finegoldii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
NADH K0.5 value, Hill coefficient 2.4, pH 8, 30°C Alistipes finegoldii
0.457
-
crotonoyl-CoA K0.5 value, Hill coefficient 1.5, pH 8, 30°C Alistipes finegoldii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
141000
-
sedimentation velocity analysis Alistipes finegoldii
145000
-
gel filtration Alistipes finegoldii

Organism

Organism UniProt Comment Textmining
Alistipes finegoldii A0A174E195
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonoyl-CoA + NADH + H+
-
Alistipes finegoldii butanoyl-CoA + NAD+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 34000, SDS-PAGE Alistipes finegoldii

Synonyms

Synonyms Comment Organism
FabI
-
Alistipes finegoldii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
presence of NADH increases the stability of FabI to thermal denaturation by 7 degrees Alistipes finegoldii

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADPH Alistipes finegoldii
NADH protein exhibits high-affinity, cooperative binding to NADH. Presence of NADH increases the stability of FabI to thermal denaturation by 7 degrees Alistipes finegoldii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00176
-
pH 8, 30°C Alistipes finegoldii triclosan