Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Alistipes finegoldii |
Crystallization (Comment) | Organism |
---|---|
crystal structure at 1.72 A resolution, protein adopts a Rossmann fold. FabI/NADH crystal structure at 1.86 A resolution, the protein undergoes a large conformational change to participate in covering the NADH-binding pocket | Alistipes finegoldii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
triclosan | - |
Alistipes finegoldii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
NADH | K0.5 value, Hill coefficient 2.4, pH 8, 30°C | Alistipes finegoldii | |
0.457 | - |
crotonoyl-CoA | K0.5 value, Hill coefficient 1.5, pH 8, 30°C | Alistipes finegoldii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
141000 | - |
sedimentation velocity analysis | Alistipes finegoldii |
145000 | - |
gel filtration | Alistipes finegoldii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alistipes finegoldii | A0A174E195 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonoyl-CoA + NADH + H+ | - |
Alistipes finegoldii | butanoyl-CoA + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 34000, SDS-PAGE | Alistipes finegoldii |
Synonyms | Comment | Organism |
---|---|---|
FabI | - |
Alistipes finegoldii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
presence of NADH increases the stability of FabI to thermal denaturation by 7 degrees | Alistipes finegoldii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADPH | Alistipes finegoldii | |
NADH | protein exhibits high-affinity, cooperative binding to NADH. Presence of NADH increases the stability of FabI to thermal denaturation by 7 degrees | Alistipes finegoldii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00176 | - |
pH 8, 30°C | Alistipes finegoldii | triclosan |