Literature summary for 1.3.1.9 extracted from

Demissie, R.D.; Kabre, P.; Tuntland, M.L.; Fung, L.W.
An efficient and economical assay to screen for triclosan binding to FabI (2016), J. Biomol. Screen., 21, 391-398 .

Search for more literature for 1.3.1.9

Application

Application	Comment	Organism
analysis	development of a simple thermal shift assay, which does not use ACP-linked substrates, to determine the binding ability of triclosan to the enzyme's active site	Francisella tularensis subsp. tularensis

Cloned(Commentary)

Cloned (Comment)	Organism
-	Francisella tularensis subsp. tularensis

Protein Variants

Protein Variants	Comment	Organism
A92V	no shift in melting temperature in presence of both triclosan and NADP+	Francisella tularensis subsp. tularensis

Inhibitors

Inhibitors	Comment	Organism	Structure
triclosan	-	Francisella tularensis subsp. tularensis

Organism

Organism	UniProt	Comment	Textmining
Francisella tularensis subsp. tularensis	Q5NGQ3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
crotonyl-CoA + NADH + H+	-	Francisella tularensis subsp. tularensis	butyryl-CoA + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
FabI	-	Francisella tularensis subsp. tularensis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
53.2	-	wild-type, melting temperature in presence of both triclosan and NADP+. Neither triclosan nor NADP+ alone increases the Tm value	Francisella tularensis subsp. tularensis
60.2	-	wild-type, melting temperature	Francisella tularensis subsp. tularensis

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Release 2023.1 (January 2023)