Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Star(DE3) cells | Eimeria tenella |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
triclosan | potent inhibitor | Eimeria tenella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
crotonyl-CoA | in 100 mM sodium phosphate (pH 7.5) | Eimeria tenella | |
0.06 | - |
NADH | in 100 mM sodium phosphate (pH 7.5) | Eimeria tenella |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Eimeria tenella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eimeria tenella | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap metal chelating column chromatography, HiTrap SP FastFlow column chromatography, and amylose column chromatography | Eimeria tenella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NADH + H+ | - |
Eimeria tenella | butyryl-CoA + NAD+ | - |
? | |
trans-2-enoyl-ACP + NADH + H+ | - |
Eimeria tenella | acyl-ACP + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | native PAGE | Eimeria tenella |
Synonyms | Comment | Organism |
---|---|---|
ENR | - |
Eimeria tenella |
NADH-dependent enoyl reductase | - |
Eimeria tenella |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Eimeria tenella |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00006 | - |
- |
Eimeria tenella | triclosan |