Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.9 extracted from

  • Massengo-Tiasse, R.P.; Cronan, J.E.
    Vibrio cholerae FabV defines a new class of enoyl-acyl carrier protein reductase (2008), J. Biol. Chem., 283, 1308-1316.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Vibrio cholerae serotype O1

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is very resistant to triclosan Vibrio cholerae serotype O1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
NADH native enzyme, in 0.1 M sodium phosphate buffer (pH 7) Vibrio cholerae serotype O1
0.195
-
crotonyl-[acyl-carrier protein] native enzyme, in 0.1 M sodium phosphate buffer (pH 7) Vibrio cholerae serotype O1
1.178
-
crotonyl-CoA native enzyme, in 0.1 M sodium phosphate buffer (pH 7) Vibrio cholerae serotype O1
3.043
-
NADPH native enzyme, in 0.1 M sodium phosphate buffer (pH 7) Vibrio cholerae serotype O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
strain ATCC 14547
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Vivapure D maxi H spin column chromatography, and Blue Sepharose column chromatography Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + NADH + H+
-
Vibrio cholerae serotype O1 butyryl-CoA + NAD+
-
?
crotonyl-CoA + NADPH + H+ the native enzyme shows very weak activity with NADPH Vibrio cholerae serotype O1 butyryl-CoA + NADP+
-
?
crotonyl-[acyl-carrier protein] + NADH
-
Vibrio cholerae serotype O1 butyryl-[acyl-carrier protein] + NAD+
-
?

Subunits

Subunits Comment Organism
monomer gel filtration Vibrio cholerae serotype O1

Synonyms

Synonyms Comment Organism
enoyl-ACP reductase
-
Vibrio cholerae serotype O1
enoyl-acyl carrier protein reductase
-
Vibrio cholerae serotype O1
FabV
-
Vibrio cholerae serotype O1

Cofactor

Cofactor Comment Organism Structure
NADH shows a very strong preference for NADH over NADPH Vibrio cholerae serotype O1