Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Vibrio cholerae serotype O1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is very resistant to triclosan | Vibrio cholerae serotype O1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
NADH | native enzyme, in 0.1 M sodium phosphate buffer (pH 7) | Vibrio cholerae serotype O1 | |
0.195 | - |
crotonyl-[acyl-carrier protein] | native enzyme, in 0.1 M sodium phosphate buffer (pH 7) | Vibrio cholerae serotype O1 | |
1.178 | - |
crotonyl-CoA | native enzyme, in 0.1 M sodium phosphate buffer (pH 7) | Vibrio cholerae serotype O1 | |
3.043 | - |
NADPH | native enzyme, in 0.1 M sodium phosphate buffer (pH 7) | Vibrio cholerae serotype O1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
strain ATCC 14547 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Vivapure D maxi H spin column chromatography, and Blue Sepharose column chromatography | Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NADH + H+ | - |
Vibrio cholerae serotype O1 | butyryl-CoA + NAD+ | - |
? | |
crotonyl-CoA + NADPH + H+ | the native enzyme shows very weak activity with NADPH | Vibrio cholerae serotype O1 | butyryl-CoA + NADP+ | - |
? | |
crotonyl-[acyl-carrier protein] + NADH | - |
Vibrio cholerae serotype O1 | butyryl-[acyl-carrier protein] + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration | Vibrio cholerae serotype O1 |
Synonyms | Comment | Organism |
---|---|---|
enoyl-ACP reductase | - |
Vibrio cholerae serotype O1 |
enoyl-acyl carrier protein reductase | - |
Vibrio cholerae serotype O1 |
FabV | - |
Vibrio cholerae serotype O1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | shows a very strong preference for NADH over NADPH | Vibrio cholerae serotype O1 |