Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Eimeria tenella |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
crotonoyl-CoA | the addition of short-chain crotonoyl-CoA actually inhibits the oxidation of NADH by recombinant ENR in a dose-dependent manner | Eimeria tenella | |
triclosan | - |
Eimeria tenella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08832 | - |
NADH | recombinant enzyme, in the absence of enoyl-CoA as scosubstrate, in 0.1 mM Tris-HCl buffer (pH 7.2) | Eimeria tenella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eimeria tenella | A3R4P1 | - |
- |
Purification (Comment) | Organism |
---|---|
amylose-resin-based affinity chromatography | Eimeria tenella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NADH + H+ | - |
Eimeria tenella | butyryl-CoA + NAD+ | - |
? | |
dodecenoyl-CoA + NADH + H+ | - |
Eimeria tenella | dodecanoyl-CoA + NAD+ | - |
? | |
additional information | the recombinant ENR expressed in bacteria is only able to oxidize NADH, but unable to transfer the electron to enoyl-CoA, possibly due to the inappropriate folding of ENR expressed in bacteria | Eimeria tenella | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enoyl reductase | the enzyme is involved in Type II fatty acid synthesis | Eimeria tenella |
ENR | - |
Eimeria tenella |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Eimeria tenella |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00145 | - |
recombinant enzyme, in 0.1 mM Tris-HCl buffer (pH 7.2) | Eimeria tenella | triclosan |