Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
A217G | 55% of wild-type kcat | Plasmodium falciparum |
A217V | 74% of wild-type kcat | Plasmodium falciparum |
F368A | no activity | Plasmodium falciparum |
F368I | 65% of wild-type kcat | Plasmodium falciparum |
M281A | no activity | Plasmodium falciparum |
M281T | 68% of wild-type kcat | Plasmodium falciparum |
N218A | no activity | Plasmodium falciparum |
N218D | 93% of wild-type kcat | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
triclosan | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
NADH | 25°C, pH 7.4 | Plasmodium falciparum | |
0.16 | - |
trans-but-2-enoyl-[acyl-carrier protein] | 25°C, pH 7.4 | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38163 | - |
4 * 38163, MALDI-TOF mass spectrometry | Plasmodium falciparum |
38166 | - |
4 * 38166, deduced from nucleotide sequence | Plasmodium falciparum |
150000 | - |
gel filtration | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q9BH77 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-but-2-enoyl-[acyl-carrier protein] + NADH | - |
Plasmodium falciparum | butanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
trans-but-2-enoyl-[acyl-carrier protein] + NADH + H+ | - |
Plasmodium falciparum | butanoyl-[acyl-carrier protein] + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38163, MALDI-TOF mass spectrometry | Plasmodium falciparum |
tetramer | 4 * 38166, deduced from nucleotide sequence | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.62 | - |
trans-but-2-enoyl-[acyl-carrier protein] | 25°C, pH 7.4 | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000003 | - |
triclosan | 25°C, pH 7.4 | Plasmodium falciparum | |
0.00000057 | - |
triclosan | 25°C, pH 7.4, A217G mutant | Plasmodium falciparum | |
0.0000015 | - |
triclosan | 25°C, pH 7.4, N218D mutant | Plasmodium falciparum | |
0.0000021 | - |
triclosan | 25°C, pH 7.4, N218A mutant | Plasmodium falciparum | |
0.0000063 | - |
triclosan | 25°C, pH 7.4, F368I mutant | Plasmodium falciparum | |
0.0000072 | - |
triclosan | 25°C, pH 7.4, F368A mutant | Plasmodium falciparum | |
0.0000093 | - |
triclosan | 25°C, pH 7.4, M281A mutant | Plasmodium falciparum | |
0.00001 | - |
triclosan | 25°C, pH 7.4, M281T mutant | Plasmodium falciparum | |
0.00023 | - |
triclosan | 25°C, pH 7.4, A217V mutant | Plasmodium falciparum |