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Literature summary for 1.3.1.88 extracted from

  • Xing, F.; Hiley, S.L.; Hughes, T.R.; Phizicky, E.M.
    The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs (2004), J. Biol. Chem., 279, 17850-17860.
    View publication on PubMed
Search for more literature for 1.3.1.88

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil16 in tRNA + NAD(P)H + H+ Saccharomyces cerevisiae the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr 5,6-dihydrouracil16 in tRNA + NAD(P)+
-
 ?
uracil17 in tRNA + NAD(P)H + H+ Saccharomyces cerevisiae the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr 5,6-dihydrouracil17 in tRNA + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P53759
-
-

Reaction

Reaction Comment Organism Reaction ID
5,6-dihydrouracil16 in tRNA + NAD(P)+ = uracil16 in tRNA + NAD(P)H + H+ (1) Saccharomyces cerevisiae
a
5,6-dihydrouracil17 in tRNA + NAD(P)+ = uracil17 in tRNA + NAD(P)H + H+ (2) Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil16 in tRNA + NAD(P)H + H+ the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr Saccharomyces cerevisiae 5,6-dihydrouracil16 in tRNA + NAD(P)+
-
 ?
uracil16 in tRNA + NAD(P)H + H+ e.g. tRNAPhe and tRNATyr Saccharomyces cerevisiae 5,6-dihydrouracil16 in tRNA + NAD(P)+
-
 ?
uracil17 in tRNA + NAD(P)H + H+ the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr Saccharomyces cerevisiae 5,6-dihydrouracil17 in tRNA + NAD(P)+
-
 ?
uracil17 in tRNA + NAD(P)H + H+ e.g. tRNAPhe and tRNATyr Saccharomyces cerevisiae 5,6-dihydrouracil17 in tRNA + NAD(P)+
-
 ?

Synonyms

Synonyms Comment Organism
Dus1p
-
 Saccharomyces cerevisiae
tRNA-dihydrouridine synthase 1
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction tRNAPhe from dus1-DELTA strain has little detectable dihydrouridine compared to tRNAPhe from wild-type. tRNAPhe contains dihydrouridine at position 16 and 17 Saccharomyces cerevisiae
physiological function formation of dihydrouridine in yeast cytoplasmic tRNAs is carried out by a family of four dihydrouridine synthases (Dus1p, Dus2p, Dus3p, and Dus4p), each acting at specific positions in tRNAs. Dus1p modifies U16 and U17, Dus2p modifies U20, Dus3p modifies U47, and Dus4p modifies U20a and U20b. These four proteins are responsible for all dihydrouridine modification of cytoplasmic tRNAs in yeast Saccharomyces cerevisiae