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Literature summary for 1.3.1.86 extracted from

  • Maitra, S.K.; Kumar, S.
    Crotonyl coenzyme A reductase activity of bovine mammary fatty acid synthetase (1974), J. Biol. Chem., 249, 111-117.
    View publication on PubMed

General Stability

General Stability Organism
the crotonyl-CoA reductase activity is influenced by preincubation. 0.25 M Tris-HCl buffer diminishes the crotonyl-CoA reductase activity by approximately 30% Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol at high concentrations beta-mercaptoethanol is inhibitory Bos taurus
dithiothreitol at high concentrations dithiothreitol is inhibitory Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
trans-crotonyl-CoA in 200 mM potassium phosphate buffer, pH 6.8, at 26°C Bos taurus
0.00364
-
NADPH in 200 mM potassium phosphate buffer, pH 6.8, at 26°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, calcium phosphate gel treatment, alumina gel Cgamma extract filtration, Sephadex G-200 gel filtration Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0061
-
crude extract, pH 6.8, at 26°C Bos taurus
0.0501
-
after 11.2fold purification, pH 6.8, at 26°C Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the crotonyl-CoA reductase reaction requires NADPH as electron donor, but at a 20fold higher concentration NADH will substitute for NADPH with 50% Vmax Bos taurus ?
-
?
trans-crotonyl-CoA + NADPH + H+ the crotonyl-CoA reductase reaction requires NADPH as electron donor Bos taurus butyryl-CoA + NADP+
-
?

Synonyms

Synonyms Comment Organism
crotonyl coenzyme A reductase
-
Bos taurus
trans-crotonyl CoA reductase
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADPH the crotonyl-CoA reductase reaction requires NADPH as electron donor, but at a 20fold higher concentration NADH will substitute for NADPH with 50% Vmax Bos taurus