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Literature summary for 1.3.1.85 extracted from

  • Erb, T.J.; Brecht, V.; Fuchs, G.; Muller, M.; Alber, B.E.
    Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase (2009), Proc. Natl. Acad. Sci. USA, 106, 8871-8876.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.85

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 CO2 at pH 7.8, temperature not specified in the publication Cereibacter sphaeroides
0.2
-
 (E)-crotonyl-CoA for reduction, pH and temperature not specified in the publication Cereibacter sphaeroides
0.4
-
 (E)-crotonyl-CoA for reductive carboxylation, pH and temperature not specified in the publication Cereibacter sphaeroides
0.5
-
 acryloyl-CoA pH and temperature not specified in the publication Cereibacter sphaeroides
0.7
-
 NADPH pH and temperature not specified in the publication Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, gel filtration Cereibacter sphaeroides
105000
-
 gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E)-crotonyl-CoA + CO2 + NADPH + H+ Cereibacter sphaeroides 100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction (2S)-ethylmalonyl-CoA + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-crotonyl-CoA + CO2 + NADPH + H+ 100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction Cereibacter sphaeroides (2S)-ethylmalonyl-CoA + NADP+
-
 r
(E)-crotonyl-CoA + NADPH + H+ 10% of maximal activity (compared with vmax of crotonyl-CoA carboxylation) Cereibacter sphaeroides butyryl-CoA + NADP+
-
 ?
acryloyl-CoA + CO2 + NADPH + H+ acryloyl-CoA is accepted as an alternative substrate analogue by the enzyme with 40% relative activity (compared with vmax of crotonyl-CoA carboxylation) Cereibacter sphaeroides methylmalonyl-CoA + NADP+
-
 ?
additional information does not reduce crotonyl-N-acetylcysteamine, methacryloyl-CoA, 6-hydroxycylohex-1-ene-1-carboxyl-CoA, cyclo-hexa-1,5-diene-1-carboxyl-CoA, acetoacetyl-CoA, propionyl-CoA, (R)-3-hydroxybutyryl-CoA, (S)-3-hydroxybutyryl-CoA (less than 1% specific activity) Cereibacter sphaeroides ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 47000, gel filtration Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
CCR
-
 Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Cereibacter sphaeroides