Application | Comment | Organism |
---|---|---|
synthesis | successful reconstitution a portion of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula in Escherichia coli to produce acrylic acid and propionic acid, involving the acryloyl-CoA reductase (ACR), overview | Metallosphaera sedula |
Protein Variants | Comment | Organism |
---|---|---|
additional information | to construct an acrylic acid-producing pathway in Escherichia coli strain MG1655DELTArecADELTAendA(DE3)/pZL42, heterologous expression of malonyl-CoA reductase (MCR), malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and 3-hydroxypropionyl-CoA dehydratase (3HPCD) Metallosphaera sedula is accompanied by recombinant overexpression of succinyl-CoA synthetase (SCS) from Escherichia coli. The engineered strain produces 13.28 mg/l of acrylic acid. To construct a propionic acid-producing pathway, the same five genes are expressed, with the addition of Metallosphaera sedula acryloyl-CoA reductase (ACR). The engineered strain produces 1430 mg/l of propionic acid, detailed overview | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylyl-CoA + NADPH + H+ | Metallosphaera sedula | - |
propanoyl-CoA + NADP+ | - |
? | |
acrylyl-CoA + NADPH + H+ | Metallosphaera sedula DSM 5348 | - |
propanoyl-CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | A4YGN2 | - |
- |
Metallosphaera sedula DSM 5348 | A4YGN2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylyl-CoA + NADPH + H+ | - |
Metallosphaera sedula | propanoyl-CoA + NADP+ | - |
? | |
acrylyl-CoA + NADPH + H+ | - |
Metallosphaera sedula DSM 5348 | propanoyl-CoA + NADP+ | - |
? | |
additional information | the purified proteins of malonyl-CoA reductase (MCR) and 3-hydroxypropionyl-CoA dehydratase (3HPCD) from Sulfolobus tokodaii, and the proteins of malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and acryloyl-CoA reductase (ACR) from Metallosphaera sedula are used to convert malonyl-CoA to propionyl-CoA in vitro. Malonyl-CoA is almost completely converted to propionyl-CoA even at 23°C, and no other acyl-CoA products are detected, indicating that this system is robust. The turnover rate of this pathway is 37/min at 37°C | Metallosphaera sedula | ? | - |
? | |
additional information | the purified proteins of malonyl-CoA reductase (MCR) and 3-hydroxypropionyl-CoA dehydratase (3HPCD) from Sulfolobus tokodaii, and the proteins of malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and acryloyl-CoA reductase (ACR) from Metallosphaera sedula are used to convert malonyl-CoA to propionyl-CoA in vitro. Malonyl-CoA is almost completely converted to propionyl-CoA even at 23°C, and no other acyl-CoA products are detected, indicating that this system is robust. The turnover rate of this pathway is 37/min at 37°C | Metallosphaera sedula DSM 5348 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Acr | - |
Metallosphaera sedula |
acryloyl-CoA reductase | - |
Metallosphaera sedula |
Msed_1426 | - |
Metallosphaera sedula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Metallosphaera sedula |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is part of the 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation cycle, pahway overview | Metallosphaera sedula |