Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | dependent on | Ruegeria pomeroyi |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta2 (DE3) cells | Escherichia coli |
expressed in Escherichia coli Rosetta2 (DE3) cells | Cereibacter sphaeroides |
expressed in Escherichia coli Rosetta2 (DE3) cells | Ruegeria pomeroyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
acrylyl-CoA | at pH 7.0 and 30°C | Escherichia coli | |
0.0015 | - |
acrylyl-CoA | at pH 7.0 and 30°C | Cereibacter sphaeroides | |
0.0028 | - |
acrylyl-CoA | at pH 7.0 and 30°C | Ruegeria pomeroyi | |
0.018 | - |
NADPH | at pH 7.0 and 30°C | Ruegeria pomeroyi | |
0.028 | - |
NADPH | at pH 7.0 and 30°C | Cereibacter sphaeroides | |
0.033 | - |
NADPH | at pH 7.0 and 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Escherichia coli | |
Mg2+ | dependent on | Cereibacter sphaeroides | |
Mg2+ | dependent on | Ruegeria pomeroyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Escherichia coli |
37000 | - |
2 * 37000, SDS-PAGE | Cereibacter sphaeroides |
37000 | - |
2 * 37000, SDS-PAGE | Ruegeria pomeroyi |
56000 | - |
gel filtration | Escherichia coli |
62000 | - |
gel filtration | Ruegeria pomeroyi |
64000 | - |
gel filtration | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylyl-CoA + NADPH + H+ | Escherichia coli | - |
propionyl-CoA + NADP+ | - |
? | |
acrylyl-CoA + NADPH + H+ | Cereibacter sphaeroides | - |
propionyl-CoA + NADP+ | - |
? | |
acrylyl-CoA + NADPH + H+ | Ruegeria pomeroyi | - |
propionyl-CoA + NADP+ | - |
? | |
acrylyl-CoA + NADPH + H+ | Cereibacter sphaeroides DSM 158 | - |
propionyl-CoA + NADP+ | - |
? | |
additional information | Escherichia coli | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | ? | - |
? | |
additional information | Cereibacter sphaeroides | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | ? | - |
? | |
additional information | Ruegeria pomeroyi | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | ? | - |
? | |
additional information | Escherichia coli | the enzyme shows less than 1% activity with crotonyl-CoA, 3-hydroxypropionyl-CoA and acrylate | ? | - |
? | |
additional information | Cereibacter sphaeroides | the enzyme shows less than 1% activity with crotonyl-CoA, 3-hydroxypropionyl-CoA and acrylate | ? | - |
? | |
additional information | Ruegeria pomeroyi | the enzyme shows less than 1% activity with crotonyl-CoA, 3-hydroxypropionyl-CoA and acrylate | ? | - |
? | |
additional information | Cereibacter sphaeroides DSM 158 | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | ? | - |
? | |
additional information | Cereibacter sphaeroides DSM 158 | the enzyme shows less than 1% activity with crotonyl-CoA, 3-hydroxypropionyl-CoA and acrylate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | Q3J6K9 | - |
- |
Cereibacter sphaeroides DSM 158 | Q3J6K9 | - |
- |
Escherichia coli | - |
- |
- |
Ruegeria pomeroyi | Q5LS56 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superose 12 gel filtration | Escherichia coli |
Ni-NTA column chromatography and Superose 12 gel filtration | Cereibacter sphaeroides |
Ni-NTA column chromatography and Superose 12 gel filtration | Ruegeria pomeroyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
cell extract, at pH 7.0 and 30°C | Escherichia coli |
12 | - |
cell extract, at pH 7.0 and 30°C | Cereibacter sphaeroides |
23 | - |
cell extract, at pH 7.0 and 30°C | Ruegeria pomeroyi |
72 | - |
after 6fold purification, at pH 7.0 and 30°C | Escherichia coli |
98 | - |
after 4fold purification, at pH 7.0 and 30°C | Ruegeria pomeroyi |
130 | - |
after 11fold purification, at pH 7.0 and 30°C | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylyl-CoA + NADPH + H+ | - |
Escherichia coli | propionyl-CoA + NADP+ | - |
? | |
acrylyl-CoA + NADPH + H+ | - |
Cereibacter sphaeroides | propionyl-CoA + NADP+ | - |
? | |
acrylyl-CoA + NADPH + H+ | - |
Ruegeria pomeroyi | propionyl-CoA + NADP+ | - |
? | |
acrylyl-CoA + NADPH + H+ | - |
Cereibacter sphaeroides DSM 158 | propionyl-CoA + NADP+ | - |
? | |
additional information | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | Escherichia coli | ? | - |
? | |
additional information | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | Cereibacter sphaeroides | ? | - |
? | |
additional information | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | Ruegeria pomeroyi | ? | - |
? | |
additional information | the enzyme shows less than 1% activity with crotonyl-CoA, 3-hydroxypropionyl-CoA and acrylate | Escherichia coli | ? | - |
? | |
additional information | the enzyme shows less than 1% activity with crotonyl-CoA, 3-hydroxypropionyl-CoA and acrylate | Cereibacter sphaeroides | ? | - |
? | |
additional information | the enzyme shows less than 1% activity with crotonyl-CoA, 3-hydroxypropionyl-CoA and acrylate | Ruegeria pomeroyi | ? | - |
? | |
additional information | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | Cereibacter sphaeroides DSM 158 | ? | - |
? | |
additional information | the enzyme shows less than 1% activity with crotonyl-CoA, 3-hydroxypropionyl-CoA and acrylate | Cereibacter sphaeroides DSM 158 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37000, SDS-PAGE | Escherichia coli |
homodimer | 2 * 37000, SDS-PAGE | Cereibacter sphaeroides |
homodimer | 2 * 37000, SDS-PAGE | Ruegeria pomeroyi |
Synonyms | Comment | Organism |
---|---|---|
acrylyl-CoA reductase | - |
Escherichia coli |
acrylyl-CoA reductase | - |
Cereibacter sphaeroides |
acrylyl-CoA reductase | - |
Ruegeria pomeroyi |
acrylyl-coenzyme A reductase | - |
Escherichia coli |
acrylyl-coenzyme A reductase | - |
Cereibacter sphaeroides |
acrylyl-coenzyme A reductase | - |
Ruegeria pomeroyi |
AcuI | - |
Escherichia coli |
AcuI | - |
Cereibacter sphaeroides |
AcuI | - |
Ruegeria pomeroyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
45 | - |
acrylyl-CoA | at pH 7.0 and 30°C | Escherichia coli | |
60 | - |
acrylyl-CoA | at pH 7.0 and 30°C | Ruegeria pomeroyi | |
80 | - |
acrylyl-CoA | at pH 7.0 and 30°C | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Cereibacter sphaeroides | |
NADPH | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | Escherichia coli | |
NADPH | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | Cereibacter sphaeroides | |
NADPH | the enzyme is highly specific for NADPH with catalytic efficiencies of more than 10fold higher for NADPH than for NADH | Ruegeria pomeroyi |