Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: EDTA, Zn2+, other divalent cations | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
acryloyl-CoA | pH 6.0, 65°C | Sulfurisphaera tokodaii | |
0.036 | - |
NADPH | pH 6.0, 65°C | Sulfurisphaera tokodaii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Sulfurisphaera tokodaii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | presence of 0.8 mol Zn2+ per mol of monomer, addition of Zn2+ is not stimulatory | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38600 | - |
1 * 39000, SDS-PAGE, 1 * 38600, calculated | Sulfurisphaera tokodaii |
39000 | - |
1 * 39000, SDS-PAGE, 1 * 38600, calculated | Sulfurisphaera tokodaii |
40000 | - |
x * 40000, SDS-PAGE | Metallosphaera sedula |
43000 | - |
gel filtration | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | - |
- |
- |
Sulfurisphaera tokodaii | Q975C8 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.9 | - |
65°C | Metallosphaera sedula |
18.7 | - |
pH 6.0, 65°C | Sulfurisphaera tokodaii |
Storage Stability | Organism |
---|---|
-20°C, 30% glycerol, stable for months | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acryloyl-CoA + NADPH + H+ | - |
Sulfurisphaera tokodaii | propanoyl-CoA + NADP+ | - |
? | |
acrylyl-CoA + NADPH + H+ | - |
Metallosphaera sedula | propanoyl-CoA + NADP+ | - |
? | |
additional information | no substrate: NADH, crotonyl-CoA | Sulfurisphaera tokodaii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Metallosphaera sedula |
monomer | 1 * 39000, SDS-PAGE, 1 * 38600, calculated | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
acryloyl-coenzyme A reductase | - |
Metallosphaera sedula |
acryloyl-coenzyme A reductase | - |
Sulfurisphaera tokodaii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
NADPH | pH 6.0, 65°C | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
half-maximal activity | Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | no cofactor: NADH | Sulfurisphaera tokodaii |