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Literature summary for 1.3.1.79 extracted from

  • Schenck, C.; Men, Y.; Maeda, H.
    Conserved molecular mechanism of TyrA dehydrogenase substrate specificity underlying alternative tyrosine biosynthetic pathways in plants and microbes (2017), Front. Mol. Biosci., 4, 73 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.79

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sediminispirochaeta smaragdinae

Protein Variants

Protein Variants Comment Organism
D208N mutation introduces prephenate dehydrogenase activity Sediminispirochaeta smaragdinae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.85
-
 L-arogenate mutant D208N, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae
0.9
-
 L-arogenate wild-type, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae
1.74
-
 prephenate mutant D208N, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae

Organism

Organism UniProt Comment Textmining
Sediminispirochaeta smaragdinae E1R5M5
-
-
Sediminispirochaeta smaragdinae DSM 11293 E1R5M5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate + NAD(P)+
-
 Sediminispirochaeta smaragdinae L-tyrosine + NAD(P)H + H+ + CO2
-
 ?
L-arogenate + NAD(P)+
-
 Sediminispirochaeta smaragdinae DSM 11293 L-tyrosine + NAD(P)H + H+ + CO2
-
 ?
prephenate + NAD(P)+ no substrate of wild-type, reaction of EC 1.3.1.12 Sediminispirochaeta smaragdinae 4-hydroxyphenylpyruvate + NAD(P)H + H+ + CO2
-
 ?
prephenate + NAD(P)+ no substrate of wild-type, reaction of EC 1.3.1.12 Sediminispirochaeta smaragdinae DSM 11293 4-hydroxyphenylpyruvate + NAD(P)H + H+ + CO2
-
 ?

Synonyms

Synonyms Comment Organism
Spirs_1513
-
 Sediminispirochaeta smaragdinae
tyrA
-
 Sediminispirochaeta smaragdinae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 L-arogenate mutant D208N, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae
0.86
-
 prephenate mutant D208N, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae
3.44
-
 L-arogenate wild-type, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae

Cofactor

Cofactor Comment Organism Structure
NAD+ slight preference for NAD+ over NADP+ Sediminispirochaeta smaragdinae
NADP+ slight preference for NAD+ over NADP+ Sediminispirochaeta smaragdinae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.57
-
 L-arogenate mutant D208N, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae
4
-
 L-arogenate wild-type, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae