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Literature summary for 1.3.1.78 extracted from

  • Rippert, P.; Matringe, M.
    Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana (2002), Eur. J. Biochem., 269, 4753-4761.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
TyrAAT1 overproduced in Escherichia coli Arabidopsis thaliana
TyrAAT2 overproduced in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
Arabidopsis thaliana
cis-aconitate
-
Arabidopsis thaliana
L-tyrosine
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
prephenate
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0102
-
NADP+ pH 7.5, 25°C, TyrAAT1 Arabidopsis thaliana
0.0147
-
NADP+ pH 7.5, 25°C, TyrAAT2 Arabidopsis thaliana
0.0526
-
arogenate pH 7.5, 25°C, TyrAAT1 Arabidopsis thaliana
0.0842
-
L-arogenate pH 7.5, 25°C, TyrAAT2 Arabidopsis thaliana
17
-
prephenate
-
Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
1 * 66000, recombinant TyrAAT1, SDS-PAGE Arabidopsis thaliana
67000
-
recombinant TyrAAT1, gel filtration Arabidopsis thaliana
600000
-
above, recombinant TyrAAT2 Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TyrAAT1 Arabidopsis thaliana
TyrAAT2 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
73
-
-
Arabidopsis thaliana
142
-
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate + NADP+ rapid equilibrium random mechanism in which two dead-end complexes, enzyme-NADPH-arogenate and enzyme-NADP+-tyrosine are formed Arabidopsis thaliana L-tyrosine + NADPH + CO2
-
?
additional information TyrAAT1 is four times more efficient in catalyzing arogenate dehydrogenase reaction than TyrAAT2. TyrAAT2 presents a weak prephenate dehydrogenase activity whereas Tyr-AAT1 does not Arabidopsis thaliana ?
-
?
prephenate + NADP+
-
Arabidopsis thaliana 3-(4-hydroxyphenyl)-2-oxopropanoate + CO2 + NADPH
-
?

Subunits

Subunits Comment Organism
monomer 1 * 66000, recombinant TyrAAT1, SDS-PAGE Arabidopsis thaliana
oligomer recombinant TyrAAT2 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
arogenate dehydrogenase isoform 1
-
Arabidopsis thaliana
arogenate dehydrogenase isoform 2
-
Arabidopsis thaliana
TyrAAT
-
Arabidopsis thaliana
TyrAAT1
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
prephenate
-
Arabidopsis thaliana
37.3
-
L-arogenate pH 7.5, 25°C, TyrAAT2 Arabidopsis thaliana
84.2
-
arogenate pH 7.5, 25°C, TyrAAT1 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0075
-
L-tyrosine pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 Arabidopsis thaliana
0.0081
-
L-tyrosine pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 Arabidopsis thaliana
0.0142
-
L-tyrosine pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 Arabidopsis thaliana
0.0166
-
L-tyrosine pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 Arabidopsis thaliana
0.0539
-
NADPH pH 7.5, 25°C, with 0.02 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 Arabidopsis thaliana
0.0588
-
NADPH pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 Arabidopsis thaliana
2.4
-
prephenate pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 Arabidopsis thaliana
4.2
-
prephenate pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 Arabidopsis thaliana
5.8
-
cis-aconitate pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 Arabidopsis thaliana
17.8
-
AMP pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 Arabidopsis thaliana
20.2
-
prephenate pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 Arabidopsis thaliana
22.5
-
prephenate pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 Arabidopsis thaliana
25.3
-
cis-aconitate pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 Arabidopsis thaliana
25.9
-
AMP pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 Arabidopsis thaliana
27.7
-
cis-aconitate pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 Arabidopsis thaliana
42.9
-
cis-aconitate pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 Arabidopsis thaliana
73.9
-
AMP pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 Arabidopsis thaliana
115
-
AMP pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 Arabidopsis thaliana