Cloned (Comment) | Organism |
---|---|
TyrAAT1 overproduced in Escherichia coli | Arabidopsis thaliana |
TyrAAT2 overproduced in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Arabidopsis thaliana | |
cis-aconitate | - |
Arabidopsis thaliana | |
L-tyrosine | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana | |
prephenate | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0102 | - |
NADP+ | pH 7.5, 25°C, TyrAAT1 | Arabidopsis thaliana | |
0.0147 | - |
NADP+ | pH 7.5, 25°C, TyrAAT2 | Arabidopsis thaliana | |
0.0526 | - |
arogenate | pH 7.5, 25°C, TyrAAT1 | Arabidopsis thaliana | |
0.0842 | - |
L-arogenate | pH 7.5, 25°C, TyrAAT2 | Arabidopsis thaliana | |
17 | - |
prephenate | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
1 * 66000, recombinant TyrAAT1, SDS-PAGE | Arabidopsis thaliana |
67000 | - |
recombinant TyrAAT1, gel filtration | Arabidopsis thaliana |
600000 | - |
above, recombinant TyrAAT2 | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TyrAAT1 | Arabidopsis thaliana |
TyrAAT2 | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
73 | - |
- |
Arabidopsis thaliana |
142 | - |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arogenate + NADP+ | rapid equilibrium random mechanism in which two dead-end complexes, enzyme-NADPH-arogenate and enzyme-NADP+-tyrosine are formed | Arabidopsis thaliana | L-tyrosine + NADPH + CO2 | - |
? | |
additional information | TyrAAT1 is four times more efficient in catalyzing arogenate dehydrogenase reaction than TyrAAT2. TyrAAT2 presents a weak prephenate dehydrogenase activity whereas Tyr-AAT1 does not | Arabidopsis thaliana | ? | - |
? | |
prephenate + NADP+ | - |
Arabidopsis thaliana | 3-(4-hydroxyphenyl)-2-oxopropanoate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, recombinant TyrAAT1, SDS-PAGE | Arabidopsis thaliana |
oligomer | recombinant TyrAAT2 | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
arogenate dehydrogenase isoform 1 | - |
Arabidopsis thaliana |
arogenate dehydrogenase isoform 2 | - |
Arabidopsis thaliana |
TyrAAT | - |
Arabidopsis thaliana |
TyrAAT1 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
prephenate | - |
Arabidopsis thaliana | |
37.3 | - |
L-arogenate | pH 7.5, 25°C, TyrAAT2 | Arabidopsis thaliana | |
84.2 | - |
arogenate | pH 7.5, 25°C, TyrAAT1 | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
L-tyrosine | pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
0.0081 | - |
L-tyrosine | pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
0.0142 | - |
L-tyrosine | pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
0.0166 | - |
L-tyrosine | pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
0.0539 | - |
NADPH | pH 7.5, 25°C, with 0.02 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
0.0588 | - |
NADPH | pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
2.4 | - |
prephenate | pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
4.2 | - |
prephenate | pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
5.8 | - |
cis-aconitate | pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
17.8 | - |
AMP | pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
20.2 | - |
prephenate | pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
22.5 | - |
prephenate | pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
25.3 | - |
cis-aconitate | pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
25.9 | - |
AMP | pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
27.7 | - |
cis-aconitate | pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
42.9 | - |
cis-aconitate | pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 | Arabidopsis thaliana | |
73.9 | - |
AMP | pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 | Arabidopsis thaliana | |
115 | - |
AMP | pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 | Arabidopsis thaliana |