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Literature summary for 1.3.1.76 extracted from

  • Stroupe, M.E.; Leech, H.K.; Daniels, D.S.; Warren, M.J.; Getzoff, E.D.
    CysG structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis (2003), Nat. Struct. Biol., 10, 1064-1073.
    View publication on PubMed
Search for more literature for 1.3.1.76

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly overproduced in Escherichia coli Salmonella enterica

Crystallization (Commentary)

Crystallization (Comment) Organism
orthorhombic space group P2(1)2(1)2(1), unit cell dimensions a = 60.73 A, b = 121.49 A, c = 130.79 A Salmonella enterica

Protein Variants

Protein Variants Comment Organism
K270I site-directed mutagenesis Salmonella enterica
L250A site-directed mutagenesis Salmonella enterica
N385A site-directed mutagenesis Salmonella enterica
S128A site-directed mutagenesis Salmonella enterica
S128D site-directed mutagenesis Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000 Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
precorrin-2 + NAD+ Salmonella enterica tetrapyrrole and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III sirohydrochlorin + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Salmonella enterica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00088
-
 dehydrogenase activity, mutant S128D Salmonella enterica
0.00993
-
 dehydrogenase activity, wild-type Salmonella enterica
0.0332
-
 dehydrogenase activity, mutant S128A Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information large multifunctional protein that catalyzes four diverse reactions, 2 S-adensyl-L-methionine-dependent methylations, NAD+-dependent tetrapyrrole dehydrogenation and metal chelation Salmonella enterica ?
-
 ?
precorrin-2 + NAD+
-
 Salmonella enterica sirohydrochlorin + NADH + H+
-
 ?
precorrin-2 + NAD+ tetrapyrrole and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III Salmonella enterica sirohydrochlorin + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 50000 Salmonella enterica

Synonyms

Synonyms Comment Organism
CysG
-
 Salmonella enterica
EC 2.1.1.107 multifunctional protein, first of three steps leading to formation of siroheme from uroporphyrinogen III Salmonella enterica
S-adenosyl-L-methionine(SAM)-dependent bismethyltransferase, dehydrogenase and ferrochelatase
-
 Salmonella enterica