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Literature summary for 1.3.1.76 extracted from

  • Schubert, H.L.; Raux, E.; Brindley, A.A.; Leech, H.K.; Wilson, K.S.; Hill, C.P.; Warren, M.J.
    The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase (2002), EMBO J., 21, 2068-2075.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.76

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
space group C2, a : 156.1 A,b : 81.2 A, c : 104.2 A Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D141A site-directed mutagenesis Saccharomyces cerevisiae
G22D site-directed mutagenesis Saccharomyces cerevisiae
H237A site-directed mutagenesis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
precorrin-2 + NAD+ Saccharomyces cerevisiae
-
 sirohydrochlorin + NADH + H+
-
 ?
precorrin-2 + NAD+ Saccharomyces cerevisiae bifunctional dehydrogenase and ferrochelatase, second of three steps leading to formation of siroheme from uroporphyrinogen III sirohydrochlorin + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P15807
-
-
Saccharomyces cerevisiae P15807 yeast
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0112
-
 wild-type Saccharomyces cerevisiae
0.0133
-
 mutant H237A Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
precorrin-2 + NAD+
-
 Saccharomyces cerevisiae sirohydrochlorin + NADH + H+
-
 ?
precorrin-2 + NAD+ bifunctional dehydrogenase and ferrochelatase, second of three steps leading to formation of siroheme from uroporphyrinogen III Saccharomyces cerevisiae sirohydrochlorin + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Met8p
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+ beta-NAD+-dependent dehydrogenation of precorrin-2 Saccharomyces cerevisiae